Document Detail


Hyperosmotic Stress Induces Cell Death in an Odontoblast-lineage Cell Line.
MedLine Citation:
PMID:  22703656     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
INTRODUCTION: Osmotic stress is one of the stimulations related to dental pain caused by caries or dentin hypersensitivity. The mechanism of osmotic-induced dental pain is not completely understood. The purpose of this study was to examine the responses of odontoblasts under sucrose-induced hyperosmotic stress.
METHODS: We used an odontoblast-lineage cell (OLC) line in our experiments. OLCs were stimulated with sucrose to produce hyperosmotic stress. The expressions of dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) were detected by using reverse transcriptase polymerase chain reaction assay. The cell viability of OLCs was detected by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assay. The responses accompanied with cell death were detected by using 4-6-diamidino-2-phenylindole staining, Western blotting of caspase-3, and annexin V assay. The expression of mitogen-activated protein kinases (MAPKs) was detected by using Western blot analysis.
RESULTS: DSPP and DMP 1 were not affected by hyperosmotic stress in OLCs. Cell viability decreased over 700 mOsm for 3 hours of cell culture. The shapes of cells and nuclei became irregular and vacuolar under hyperosmotic stress. The expression of cleaved caspase-3 was increased after treatment with hyperosmotic stress. Some propidium iodide-positive cells were detected in flow cytometry analysis. Phosphorylation of 3 MAPKs was induced by hyperosmotic stress. Inhibitors of 3 MAPKs inhibited the hyperosmotic stress-induced decline in cell viability at 500 and 700 mOsm.
CONCLUSIONS: Hyperosmotic stress induces cell death of OLCs with sucrose through a MAPK pathway.
Authors:
Mari Fujisawa; Masayuki Tokuda; Yoko Morimoto-Yamashita; Shoko Tatsuyama; Szilvia Arany; Toshihiro Sugiyama; Chiaki Kitamura; Yoshiyuki Shibukawa; Mitsuo Torii
Related Documents :
18988316 - Merkel cell carcinoma within follicular cysts: report of two cases.
20211016 - Cd40 ligand induced cytotoxicity in carcinoma cells is enhanced by inhibition of metall...
6265846 - Primary small cell ("oat cell") carcinoma of the larynx and hypopharynx.
2120896 - Ultrastructure and neuron-specific enolase (nse) immunohistochemistry of merkel cells i...
15466566 - Stratified growth in pseudomonas aeruginosa biofilms.
7854336 - A transformed murine leydig cell line expresses the eta receptor subtype.
Publication Detail:
Type:  Journal Article     Date:  2012-05-05
Journal Detail:
Title:  Journal of endodontics     Volume:  38     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  931-5     Citation Subset:  D    
Copyright Information:
Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Antimicrobial Substantivity over Time of Chlorhexidine and Cetrimide.
Next Document:  Effects of an Experimental Calcium Aluminosilicate Cement on the Viability of Murine Odontoblast-lik...