| Hyperosmotic Stress Induces Cell Death in an Odontoblast-lineage Cell Line. | |
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MedLine Citation:
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PMID: 22703656 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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INTRODUCTION: Osmotic stress is one of the stimulations related to dental pain caused by caries or dentin hypersensitivity. The mechanism of osmotic-induced dental pain is not completely understood. The purpose of this study was to examine the responses of odontoblasts under sucrose-induced hyperosmotic stress. METHODS: We used an odontoblast-lineage cell (OLC) line in our experiments. OLCs were stimulated with sucrose to produce hyperosmotic stress. The expressions of dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) were detected by using reverse transcriptase polymerase chain reaction assay. The cell viability of OLCs was detected by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assay. The responses accompanied with cell death were detected by using 4-6-diamidino-2-phenylindole staining, Western blotting of caspase-3, and annexin V assay. The expression of mitogen-activated protein kinases (MAPKs) was detected by using Western blot analysis. RESULTS: DSPP and DMP 1 were not affected by hyperosmotic stress in OLCs. Cell viability decreased over 700 mOsm for 3 hours of cell culture. The shapes of cells and nuclei became irregular and vacuolar under hyperosmotic stress. The expression of cleaved caspase-3 was increased after treatment with hyperosmotic stress. Some propidium iodide-positive cells were detected in flow cytometry analysis. Phosphorylation of 3 MAPKs was induced by hyperosmotic stress. Inhibitors of 3 MAPKs inhibited the hyperosmotic stress-induced decline in cell viability at 500 and 700 mOsm. CONCLUSIONS: Hyperosmotic stress induces cell death of OLCs with sucrose through a MAPK pathway. |
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Authors:
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Mari Fujisawa; Masayuki Tokuda; Yoko Morimoto-Yamashita; Shoko Tatsuyama; Szilvia Arany; Toshihiro Sugiyama; Chiaki Kitamura; Yoshiyuki Shibukawa; Mitsuo Torii |
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Publication Detail:
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Type: Journal Article Date: 2012-05-05 |
Journal Detail:
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Title: Journal of endodontics Volume: 38 ISSN: 1878-3554 ISO Abbreviation: J Endod Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-06-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: United States |
Other Details:
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Languages: eng Pagination: 931-5 Citation Subset: D |
Copyright Information:
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Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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