Document Detail

Hyperosmotic Stress Induces Cell Death in an Odontoblast-lineage Cell Line.
MedLine Citation:
PMID:  22703656     Owner:  NLM     Status:  In-Data-Review    
INTRODUCTION: Osmotic stress is one of the stimulations related to dental pain caused by caries or dentin hypersensitivity. The mechanism of osmotic-induced dental pain is not completely understood. The purpose of this study was to examine the responses of odontoblasts under sucrose-induced hyperosmotic stress.
METHODS: We used an odontoblast-lineage cell (OLC) line in our experiments. OLCs were stimulated with sucrose to produce hyperosmotic stress. The expressions of dentin sialophosphoprotein (DSPP) and dentin matrix protein 1 (DMP 1) were detected by using reverse transcriptase polymerase chain reaction assay. The cell viability of OLCs was detected by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assay. The responses accompanied with cell death were detected by using 4-6-diamidino-2-phenylindole staining, Western blotting of caspase-3, and annexin V assay. The expression of mitogen-activated protein kinases (MAPKs) was detected by using Western blot analysis.
RESULTS: DSPP and DMP 1 were not affected by hyperosmotic stress in OLCs. Cell viability decreased over 700 mOsm for 3 hours of cell culture. The shapes of cells and nuclei became irregular and vacuolar under hyperosmotic stress. The expression of cleaved caspase-3 was increased after treatment with hyperosmotic stress. Some propidium iodide-positive cells were detected in flow cytometry analysis. Phosphorylation of 3 MAPKs was induced by hyperosmotic stress. Inhibitors of 3 MAPKs inhibited the hyperosmotic stress-induced decline in cell viability at 500 and 700 mOsm.
CONCLUSIONS: Hyperosmotic stress induces cell death of OLCs with sucrose through a MAPK pathway.
Mari Fujisawa; Masayuki Tokuda; Yoko Morimoto-Yamashita; Shoko Tatsuyama; Szilvia Arany; Toshihiro Sugiyama; Chiaki Kitamura; Yoshiyuki Shibukawa; Mitsuo Torii
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Publication Detail:
Type:  Journal Article     Date:  2012-05-05
Journal Detail:
Title:  Journal of endodontics     Volume:  38     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  931-5     Citation Subset:  D    
Copyright Information:
Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Department of Restorative Dentistry and Endodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
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