Document Detail


Hyperosmolaric contrast agents in cartilage tomography may expose cartilage to overload-induced cell death.
MedLine Citation:
PMID:  22206829     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In clinical arthrographic examination, strong hypertonic contrast agents are injected directly into the joint space. This may reduce the stiffness of articular cartilage, which is further hypothesized to lead to overload-induced cell death. We investigated the cell death in articular cartilage while the tissue was compressed in situ in physiological saline solution and in full strength hypertonic X-ray contrast agent Hexabrix(TM). Samples were prepared from bovine patellae and stored in Dulbecco's Modified Eagle's Medium overnight. Further, impact tests with or without creep were conducted for the samples with contact stresses and creep times changing from 1MPa to 10MPa and from 0min to 15min, respectively. Finally, depth-dependent cell viability was assessed with a confocal microscope. In order to characterize changes in the biomechanical properties of cartilage as a result of the use of Hexabrix™, stress-relaxation tests were conducted for the samples immersed in Hexabrix™ and phosphate buffered saline (PBS). Both dynamic and equilibrium modulus of the samples immersed in Hexabrix™ were significantly (p<0.05) lower than those of the samples immersed in PBS. Cartilage samples immersed in physiological saline solution showed load-induced cell death primarily in the superficial and middle zones. However, under high 8-10MPa contact stresses, the samples immersed in full strength Hexabrix™ showed significantly (p<0.05) higher number of dead cells than the samples compressed in physiological saline, especially in the deep zone of cartilage. In conclusion, excessive loading stresses followed by tissue creep might increase the risk for chondrocyte death in articular cartilage when immersed in hypertonic X-ray contrast agent, especially in the deep zone of cartilage.
Authors:
M J Turunen; J Töyräs; M J Lammi; J S Jurvelin; R K Korhonen
Related Documents :
2732199 - Elevation of glutathione levels and glutathione s-transferase activity in arsenic-resis...
20206609 - Inhibition of epidermal growth factor receptor-overexpressing cancer cells by camptothe...
21267699 - Hybrid sclerosing adenosis and basal cell hyperplasia of the prostate.
9472109 - Adenovirus-mediated p16 transfer to glioma cells induces g1 arrest and protects from pa...
14618629 - Reversal of breast cancer resistance protein (bcrp/abcg2)-mediated drug resistance by n...
18565219 - Altered localisation of the copper efflux transporters atp7a and atp7b associated with ...
9018259 - Altered regulation of cell surface peptidases in human cholesteatoma.
8265349 - Modified binding of proteins from calcitonin-negative tumor cells to the neuroendocrine...
1822839 - Cell aggregates of escherichia coli with benzylpenicillin amidase activity.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-27
Journal Detail:
Title:  Journal of biomechanics     Volume:  -     ISSN:  1873-2380     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0157375     Medline TA:  J Biomech     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Applied Physics, University of Eastern Finland, Kuopio, Finland; Department of Clinical Physiology, Kuopio University Hospital, Kuopio, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dependence of cyclic stretch-induced stress fiber reorientation on stretch waveform.
Next Document:  Mammographic features of screening detected pT1 (a-b) invasive breast cancer using BI-RADS lexicon.