Document Detail


Hypermorphic expression of centromeric retroelement-encoded small RNAs impairs CENP-A loading.
MedLine Citation:
PMID:  23392618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The proper functioning of centromeres requires a complex cascade of epigenetic events involving chromatin and kinetochore assembly; however, the precise mechanism by which this cascade proceeds is unknown. The pivotal event during kinetochore formation is the "loading," or deposition, of CENP-A. This histone H3 variant is specific to centromeres and replaces conventional H3 in centromeric chromatin. Failure to load CENP-A into mammalian centromeres in late telophase/early G1 of the cell cycle leads to malsegregation and cell division defects in subsequent cell cycles. Mounting evidence supports the hypothesis that an RNA component is involved, although how RNAs participate in centromere formation in mammals has remained unknown. Using the marsupial model, the tammar wallaby, we show that centromeric retroelements produce small RNAs and that hypermorphic expression of these centromeric small RNAs results in disruption of CENP-A localization. We propose that tight regulation of the processing of this new class of small RNAs, crasiRNAs, is an integral component of the epigenetic framework necessary for centromere establishment.
Authors:
Dawn M Carone; Chu Zhang; Laura E Hall; Craig Obergfell; Benjamin R Carone; Michael J O'Neill; Rachel J O'Neill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-08
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  21     ISSN:  1573-6849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-09-03     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  49-62     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantigens / genetics*,  metabolism
Centromere / genetics*
Chromatin / genetics
Chromosomal Proteins, Non-Histone / antagonists & inhibitors,  genetics*,  metabolism
Epigenesis, Genetic
Humans
Kinetochores
Macropodidae / genetics*
Mitosis / genetics
Nucleosomes / genetics
RNA, Small Interfering / genetics*,  isolation & purification
Retroelements / genetics*
Grant Support
ID/Acronym/Agency:
T32 HD007439/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Autoantigens; 0/Chromatin; 0/Chromosomal Proteins, Non-Histone; 0/Nucleosomes; 0/RNA, Small Interfering; 0/Retroelements; 0/centromere protein A

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