Document Detail

Hypermethylation of GSTP1, CD44, and E-cadherin genes in prostate cancer among US Blacks and Whites.
MedLine Citation:
PMID:  12692786     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In the US, the incidence and mortality of prostate cancer is about twofold higher among US Blacks compared to Whites, suggesting racial differences in prostate tumor occurrence and aggressiveness. The reason for these racial differences is unknown. Epigenetic events such as promoter-region gene hypermethylation may be influenced by environmental exposures and have been implicated in prostate carcinogenesis (by the silencing of tumor suppressors and other regulatory genes). METHODS: Using real-time methylation-sensitive PCR, we assessed differences in DNA hypermethylation of GSTP1, CD44, and E-cadherin (three genes thought to be important in the progression of prostate cancer) in archival tumor tissue of black (n = 47) and white men (n = 64). RESULTS: We found a high prevalence of GSTP1 hypermethylation overall (84%) but no differences by race (89 and 83% in black vs. white men, respectively), tumor stage, or grade. Although CD44 hypermethylation was less prevalent overall (found in 32% of tumors), we observed a 1.7-fold higher frequency among black men (43 vs. 25% in black vs. white men, P = 0.05) and a correlation with tumor grade (CD44 was hypermethylated in 10, 42, and 52% of well, moderate, and poorly differentiated tumors, respectively, P = 0.003) but not disease stage. The E-cadherin gene was not hypermethylated in any of the tumors. In summary, of the three genes examined, only CD44 hypermethylation differed by race and correlated with tumor grade, independent of race. CONCLUSIONS: These preliminary findings suggest that differences in gene promoter hypermethylation may potentially underlie racial differences in prostate cancer pathogenesis and should be explored in larger studies.
Karen Woodson; Richard Hayes; Louise Wideroff; Liza Villaruz; Joseph Tangrea
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Prostate     Volume:  55     ISSN:  0270-4137     ISO Abbreviation:  Prostate     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-14     Completed Date:  2003-05-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  United States    
Other Details:
Languages:  eng     Pagination:  199-205     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Cancer Prevention Studies Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.
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MeSH Terms
African Continental Ancestry Group
Antigens, CD44 / genetics*,  metabolism
Cadherins / genetics*,  metabolism
Carcinoma / genetics*
DNA Methylation*
DNA, Neoplasm / genetics,  metabolism
European Continental Ancestry Group
Glutathione S-Transferase pi
Glutathione Transferase / genetics*,  metabolism
Isoenzymes / genetics*,  metabolism
Middle Aged
Polymerase Chain Reaction / methods
Prostatic Neoplasms / genetics*
Reg. No./Substance:
0/Antigens, CD44; 0/Cadherins; 0/DNA, Neoplasm; 0/Isoenzymes; EC protein, human; EC S-Transferase pi; EC Transferase

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