| Hypermethylation of the CpG island spanning from exon II to intron III is associated with steroidogenic factor 1 expression in stromal cells of endometriosis. | |
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MedLine Citation:
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PMID: 21926385 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Unravel the mechanism for differential SF-1 expression in endometrial and endometriotic stromal cells. DESIGN: We identified a novel CpG island in the SF-1 gene, which spans from exon II to intron III. We evaluated the methylation status of this CpG island. PATIENTS: Eutopic endometrium from disease-free participants (n = 8) and the walls of cystic endometriosis lesions of the ovaries (n = 8). None of the patients had received any preoperative hormonal therapy. Stromal cells were isolated from these 2 types of tissues. RESULTS: SF-1 messenger RNA (mRNA) levels in endometriotic stromal cells were significantly higher than those in endometrial stromal cells. Bisulfite sequencing showed strikingly increased methylation in endometriotic cells compared with endometrial cells (P < .001). A strong correlation between mRNA levels and percentage methylation of the exon II/intron III are observed. Specifically, the Pearson correlation coefficient was .98 (P < .001) for this association. CONCLUSIONS: We demonstrated that methylation of a coding exon/intron sequence in the SF-1 gene positively regulated its expression in endometriosis, whereas its hypomethylation in normal endometrium was associated with drastically lower SF-1 levels. |
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Authors:
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Qing Xue; Ying Fang Zhou; Sai Nan Zhu; Serdar E Bulun |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-09-16 |
Journal Detail:
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Title: Reproductive sciences (Thousand Oaks, Calif.) Volume: 18 ISSN: 1933-7205 ISO Abbreviation: Reprod Sci Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-10-27 Completed Date: 2012-02-08 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 101291249 Medline TA: Reprod Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1080-4 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, First Hospital of Beijing University, Beijing, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cells, Cultured CpG Islands / genetics* DNA Methylation / genetics* Endometriosis / metabolism* Exons / genetics Female Gene Expression Humans Introns / genetics RNA, Messenger / analysis Real-Time Polymerase Chain Reaction Steroidogenic Factor 1 / genetics* Stromal Cells / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R37-HD38691/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/NR5A1 protein, human; 0/RNA, Messenger; 0/Steroidogenic Factor 1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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