| Hyperlipidemic chicken as a model of non-alcoholic steatohepatitis. | |
| | |
MedLine Citation:
|
PMID: 18997102 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Non-alcoholic steatohepatitis (NASH) is part of the spectrum of non-alcoholic fatty liver disease (NAFLD), currently the most common cause of abnormal liver tests. Given the difficulty of studying all the factors involved in it in human populations, studies in animal models might provide crucial insights in the pathogenesis of steatohepatitis. Several physiological features predispose birds to fat deposition in the liver. The present study was conceived to explore the possibilities of the chicken fed a cholesterol and fat enriched diet as a model for steatohepatitis. We used two different diets: a standard growing mash (control group) and a standard growing mash enriched with 2% cholesterol and 20% palm oil (hyperlipidemic group). We investigated the effect of feeding a cholesterol and fat enriched diet, on plasma lipid levels, liver enzymes and hepatic histopathology. Semiquantitative and quantitative assessment by image analysis was performed to determine changes in lipid deposits and inflammatory infiltration. Statistically significant increases were observed in all plasma lipid parameters, liver macroscopic features, fat deposits and cell-ballooning of hepatocytes between control and hyperlipidemic animals. Significant differences were also observed in the inflammatory infiltration parameters (number of foci, density, area and maximal diameter). Results show that diet-induced hypercholesterolemia and hypertriglyceridemia are associated with severe impairment of liver histology (fat accumulation, inflammation and cell-ballooning), reproducing histological features of human NAFLD. This model, which is easy and reproducible, offers economic and technical advantages. Furthermore, the reversibility of the pathologic changes makes it suitable for drug intervention studies of steatohepatitis. |
| | |
Authors:
|
Ignacio Ayala; Antonia Martín Castillo; Gracia Adánez; Ana Fernández-Rufete; Bartolomé García Pérez; Maria T Castells |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-07 |
Journal Detail:
|
Title: Experimental biology and medicine (Maywood, N.J.) Volume: 234 ISSN: 1535-3702 ISO Abbreviation: Exp. Biol. Med. (Maywood) Publication Date: 2009 Jan |
Date Detail:
|
Created Date: 2008-12-25 Completed Date: 2009-02-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100973463 Medline TA: Exp Biol Med (Maywood) Country: United States |
Other Details:
|
Languages: eng Pagination: 10-6 Citation Subset: IM |
Affiliation:
|
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain. iayape@um.es |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alanine Transaminase
/
blood Animals Aspartate Aminotransferases / blood Biological Markers / blood Chickens Cholesterol, Dietary / adverse effects Disease Models, Animal Fatty Liver / blood*, pathology Hepatocytes / pathology Hyperlipidemias / blood* Inflammation / pathology Liver / pathology Male gamma-Glutamyltransferase / blood |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/Cholesterol, Dietary; EC 2.3.2.2/gamma-Glutamyltransferase; EC 2.6.1.1/Aspartate Aminotransferases; EC 2.6.1.2/Alanine Transaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Allyl isothiocyanate influences cell adhesion, migration and metalloproteinase gene expression in SK...
Next Document: Proteomic analysis of endothelial cell autoantigens recognized by anti-dengue virus nonstructural pr...