| Hypericin incorporation and localization in fixed HeLa cells for various conditions of fixation and incubation. | |
| | |
MedLine Citation:
|
PMID: 21243156 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Hypericin is a photosensitizer expressing high affinity for cancerous cells in vivo. Diagnosis of cancer based on hypericin fluorescence imaging has been successfully assessed in several clinical trials. Our final objective will be to evaluate the potential of hypericin fluorescence imaging to improve the efficacy of cervical cancer diagnosis performed on fixed cell smears obtained from liquid-based cytology. For this purpose, the mechanism of hypericin incorporation and localization in fixed HeLa cells using different incubation media and fixation conditions was investigated. Since the duration of fixation may play an important role, the influence of fixation time on hypericin incorporation in fixed HeLa cells was studied. The uptake and distribution of hypericin in fixed HeLa cells were found to be strongly dependent on the hypericin incubation medium: for a polar organic solvent such as the alcohol-based fixative, the localization was essentially perinuclear and nuclear; for cell culture medium supplemented with serum, the localization was cytoplasmic and non-specific; the highest incorporation was observed for the serum-free culture medium but mainly as non-fluorescent aggregates. The hypericin aggregation in the incubation medium, the passive diffusion and the partitioning between the cells and hypericin carriers seemed to be the major factors accounting for these results. The localization was found to be weakly dependent on fixation time, whereas fluctuations of hypericin fluorescence at short fixation time and stabilization after two days of fixation were observed. These results suggest that the fixed cells reached a steady state after two days of fixation. |
| | |
Authors:
|
Thanh T K Vuong; Christine Vever-Bizet; Stéphanie Bonneau; Geneviève Bourg-Heckly |
Related Documents
:
|
21590716 - Antitumor effects of telomerase inhibitor tmpyp4 in osteosarcoma cell lines. 21670286 - Osmotic stress triggers toxin production by the dinoflagellate karenia brevis. 21750406 - Too many breaks (brakes): pancreatic β-cell senescence leads to diabetes. 7666166 - Paired-pulse facilitation of ipscs in slices of immature and mature mouse somatosensory... 2971656 - Mechanism of the cytotoxic synergism of fluoropyrimidines and folinic acid in mouse leu... 10906216 - Infectious hematopoietic necrosis virus matrix protein inhibits host-directed gene expr... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-1-18 |
Journal Detail:
|
Title: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Volume: - ISSN: 1474-9092 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-1-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101124451 Medline TA: Photochem Photobiol Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Université Pierre et Marie Curie, ANBioPhy-FRE CNRS 3207, Case courrier 138, 4 place Jussieu, 75005 Paris, France. chvever@mnhn.fr. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Synthesis of star-shaped monodisperse oligo(9,9-di-n-octylfluorene-2,7-vinylene)s functionalized tru...
Next Document: Supramolecular cations of the m-fluoroanilinium(dibenzo[18]crown-6) in ferromagnetic salt.