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Hyperexpression of Escherichia coli Xylose Isomerase.
MedLine Citation:
PMID:  20568206     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The xylose isomerase (xylA) structural gene was cloned under the control of the tac promoter and expressed in a xyl(+) E. coli strain. Xylose isomerase accounted for approximately 28% of the total cell protein when this tac-xylA fusion was induced with isopropylthio beta-D-galactopyranoside. Hyperexpression of the xylA gene inhibited xylose utilization. E. coli carrying this tac-xylA fusion was encapsulated in calcium-alginate beads and used to isomerase xylose in a column reactor. Conversion of xylose to xylulose was 3-4% with a residence time in the column of 2 minutes and a maximum of 12% upon recycling.
Authors:
C A Batt; E O'Neill; S R Novak; J Ko; A Sinskey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  2     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  2010-06-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-4     Citation Subset:  -    
Affiliation:
Department of Food Science, Cornell Universty, Ithaca, N. Y. 14853.
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