Document Detail


Hyperbaric whole-body plethysmograph for rodents.
MedLine Citation:
PMID:  6612899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Described is a rodent size whole-body plethysmograph constructed of two concentric Plexiglas cylinders. Chamber temperature is controlled by circulating water of constant temperature between the cylinders, the entry gas also being preconditioned to a desired temperature. An aluminum plate partitions the chamber into an animal compartment (1.4 liters without the rat) and a transducer compartment (1.1 liters). Dynamic calibration indicates the system response is uniform between 60 and 270 cycles/min. Static calibration shows a linear correlation between the transducer output (in microV) and the calibration volume. Under hyperbaric conditions such linear correlation also exists, but the transducer outputs are greater for a given calibration volume. For a given volume of injection the transducer output is a linear function of log-ambient pressure. Respiratory variables measured with this plethysmograph are comparable to those reported in the literature both for 1 ATA and under hyperbaric conditions. The plethysmograph is also adaptable for measurement of oxygen consumption and CO2 production, under elevated ambient pressure. Caution for this application is given in regard to 1) the protracted time requirement for reaching a steady state, 2) accumulation of CO2 in the chamber, and 3) diminishing gas concentration of interest between the entering and existing gases, suggesting a trade-off in selecting chamber size, ventilation rate, and level of acceptable CO2 in the chamber.
Authors:
Y C Lin; K K Shida; B L Respicio
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Undersea biomedical research     Volume:  10     ISSN:  0093-5387     ISO Abbreviation:  Undersea Biomed Res     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-10-21     Completed Date:  1983-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0421514     Medline TA:  Undersea Biomed Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  135-45     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Animals
Plethysmography, Whole Body / instrumentation*,  methods
Pressure*
Rats
Rodentia / physiology
Transducers, Pressure

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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