Document Detail

Hyperbaric oxygen therapy reduces the severity of ischaemia, preservation and reperfusion injury in a rat model of liver transplantation.
MedLine Citation:
PMID:  22221571     Owner:  NLM     Status:  MEDLINE    
BACKGROUND:   Approaches to increase organ availability for orthotopic liver transplantation (OLT) often result in the procurement of marginal livers that are more susceptible to ischaemia, preservation and reperfusion injury (IPRI).
METHODS:   The effects of post-OLT hyperbaric oxygen (HBO) therapy on IPRI in a syngeneic rat OLT model were examined at various time-points. The effects of IPRI and HBO on hepatocyte necrosis, apoptosis, proliferation, and sinusoidal morphology and ultrastructure were assessed.
RESULTS:   Post-OLT HBO therapy significantly reduced the severity of IPRI; both apoptosis [at 12 h: 6.4 ± 0.4% in controls vs. 1.6 ± 0.7% in the HBO treatment group (p < 0.001); at 48 h: 2.4 ± 0.2% in controls vs. 0.4 ± 0.1% in the HBO treatment group (p < 0.001)] and necrosis [at 12 h: 18.7 ± 1.8% in controls vs. 2.4 ± 0.4% in the HBO treatment group (p < 0.001); at 48 h: 8.5 ± 1.3% in controls vs. 3.4 ± 0.9% in the HBO treatment group (P= 0.019)] were decreased. Serum alanine transaminase was reduced [at 12 h: 1068 ± 920 IU/l in controls vs. 370 ± 63 IU/l in the HBO treatment group (P= 0.030); at 48 h: 573 ± 261 IU/l in controls vs. 160 ± 10 IU/l in the HBO treatment group (P= 0.029)]. Treatment with HBO also promoted liver regeneration [proliferation at 12 h: 4.5 ± 0.1% in controls vs. 1.0 ± 0.3% in the HBO treatment group (p < 0.001); at 48 h: 8.6 ± 0.7% in controls vs. 2.9 ± 0.2% in the HBO treatment group (p < 0.01)] and improved sinusoidal diameter and microvascular density index.
CONCLUSIONS:   Hyperbaric oxygen therapy has persistent positive effects post-OLT that may potentially transfer into clinical practice.
Nhut Quang Tran; Caterina Malcontenti-Wilson; Soukena Hammoud; Ian Millar; Chris Christophi; Vijayaragavan Muralidharan
Publication Detail:
Type:  Journal Article     Date:  2011-11-13
Journal Detail:
Title:  HPB : the official journal of the International Hepato Pancreato Biliary Association     Volume:  14     ISSN:  1477-2574     ISO Abbreviation:  HPB (Oxford)     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-06     Completed Date:  2012-05-08     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  100900921     Medline TA:  HPB (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  103-14     Citation Subset:  IM    
Copyright Information:
© 2011 International Hepato-Pancreato-Biliary Association.
Department of Surgery, The University of Melbourne, Austin Health, Studley Road, Heidelberg, Victoria, Australia.
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MeSH Terms
Biological Markers / blood
Cell Proliferation
Disease Models, Animal
Hyperbaric Oxygenation*
Liver / blood supply*,  surgery*,  ultrastructure
Liver Regeneration
Liver Transplantation / adverse effects*
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Rats, Inbred Lew
Reperfusion Injury / blood,  etiology,  pathology,  prevention & control*
Severity of Illness Index
Time Factors
Reg. No./Substance:
0/Biological Markers

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