Document Detail

Hyperbaric oxygen preconditioning alleviates myocardial ischemic injury in rats.
MedLine Citation:
PMID:  18703748     Owner:  NLM     Status:  MEDLINE    
It has been shown that after ischemia-reperfusion, application of hyperbaric oxygen (HBO) reduces cardiac injury. In this study we tested the hypothesis that HBO preconditioning reduces injury to the ischemic myocardium. One hundred and eight adult male Sprague-Dawley rats (250-280 g) were randomly divided into four groups: normoxia + sham surgery (CS), normoxia + permanent occlusion of the left anterior descending (LAD) coronary artery (CMI), HBO preconditioning + sham surgery (HS), and HBO preconditioning + permanent LAD occlusion (HMI). Rats receiving HBO preconditioning were intermittently exposed to 100% O(2) at 2.5 atmosphere absolute (ATA) for 60 min, twice daily for 2 days followed by 12 hrs of recovery in room air prior to the myocardial ischemic insult induced by LAD ligation. Rats in the normoxia group were time-matched with the HBO group and maintained under normoxic conditions prior to LAD occlusion. At 3 and 7 days after LAD occlusion, heart function parameters were measured by inserting a catheter into the left ventricle, infarct size was calculated using the method of TTC staining, myocardial capillary density was determined by immunohistochemical staining with a monoclonal anti-CD(31)/PECAM-1 antibody, and VEGF protein level was determined by Western blot analysis. At 3 days after LAD ligation, the infarct size of the HMI group was significantly smaller than that of the CMI group (26 +/- 2.5% vs. 38 +/- 3%, P < 0.05). The heart function parameters including left ventricular systolic pressure (LVSP), +dP/dt(max) and -dP/dt(max) were significantly improved in the HMI group compared to the CMI group at 3 and 7 days after LAD occlusion. Capillary density and VEGF protein levels were significantly increased in the ischemic myocardium pre-exposed to HBO. We conclude that HBO preconditioning alleviates myocardial ischemia in rat model.
Cuihong Han; Li Lin; Weidong Zhang; Li Zhang; Shijun Lv; Qiang Sun; Hengyi Tao; John H Zhang; Xuejun Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-14
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  233     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-29     Completed Date:  2009-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1448-53     Citation Subset:  IM    
Department of Diving Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.
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MeSH Terms
Capillaries / pathology
Heart Rate
Hyperbaric Oxygenation*
Myocardial Infarction / pathology,  therapy*
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A / metabolism
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A; 0/vascular endothelial growth factor A, rat

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