| Hyper-expression of small nucleolar RNAs (snoRNAs) in female inflorescences of hazelnut (Corylus avellana L.) supports rRNA aggregation in vitro. | |
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MedLine Citation:
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PMID: 14519769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Under certain in vitro (salt and temperature) conditions rRNA aggregation occurs in female inflorescences but not in leaves or pollen RNA preparations from hazelnut (Corylus avellana L.), a species of economic interest. This paper describes experiments addressing an explanation of this phenomenon. The experiments demonstrate that: (i) trans-acting factors induce rRNA aggregate formation in female inflorescences RNA preparations; (ii) these factors support aggregation also of heterologous rRNA; (iii) aggregation is a function of temperature pre-treatment of rRNA and not of source 18S rRNA; (iv) the factors inducing rRNA aggregates are sensitive to RNase; (v) antisense small nucleolar RNAs (snoRNAs) participate in rRNA aggregate formation. snoRNAs are involved in pre-rRNA spacer cleavages, and are required for the two most common types of rRNA modifications: 2'-O-ribose methylation and pseudouridylation. Even though it is questionable whether rRNA aggregation really happens in female inflorescence in vivo, the phenomenon observed in vitro may reflect the abundance of snoRNAs in these reproductive structures. In fact the level of accumulation of three tested snoRNAs, R1, U14 and U3, is much higher in female inflorescence than in leaves or pollen of hazelnut. This finding opens the possibility of studying the role of snoRNAs in tissue development in plants. |
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Authors:
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Domenica Rita Massardo; Bruno Esposito; Attilio Veneziano; Klaus Wolf; Pietro Alifano; Luigi Del Giudice |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plant & cell physiology Volume: 44 ISSN: 0032-0781 ISO Abbreviation: Plant Cell Physiol. Publication Date: 2003 Sep |
Date Detail:
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Created Date: 2003-10-01 Completed Date: 2004-02-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: Japan |
Other Details:
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Languages: eng Pagination: 884-92 Citation Subset: IM |
Affiliation:
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Istituto di Genetica e Biofisica Adriano Buzzati-Traverso--C.N.R., Via G. Marconi 10, I-80125 Napoli, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Corylus / genetics*, physiology DNA Primers Plant Components, Aerial / metabolism* RNA, Ribosomal / genetics* RNA, Small Nucleolar / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/RNA, Ribosomal; 0/RNA, Small Nucleolar |
| Comments/Corrections | |
Erratum In:
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Plant Cell Physiol. 2003 Nov;44(11):1253 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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