Document Detail

Hydroxylated and non-hydroxylated sulfatide are distinctly distributed in the human cerebral cortex.
MedLine Citation:
PMID:  21802498     Owner:  NLM     Status:  Publisher    
Sulfatide (ST) is a sphingolipid with an important role in the central nervous system as a major component of the myelin sheath. ST contains a structurally variable ceramide moiety, with a fatty acid substituent of varying carbon-chain length and double-bond number. Hydroxylation at the α-2 carbon position of the fatty acid is found in half the population of ST molecules. Recent genetic studies of fatty acid 2-hydroxylase (FA2H) indicate that these hydroxylated sphingolipids influence myelin sheath stability. However, their distribution is unknown. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) enables the analysis of distinct distributions of individual ST molecular species in tissue section. We examined human cerebral cortex tissue sections with MALDI-IMS, identifying and characterizing the distributions of 14 ST species. The distribution analysis reveals that the composition ratios of non-hydroxylated/hydroxylated STs are clearly reversed at the border between white and gray matter; the hydroxylated group is the dominant ST species in the gray matter. These results suggest that hydroxylated STs are highly expressed in oligodendrocytes in gray matter and might form stable myelin sheaths. As a clinical application, we analyzed a brain with Alzheimer's disease (AD) as a representative neurodegenerative disease. Although previous studies of AD pathology have reported that the amount of total ST is decreased in the cerebral cortex, as far as the compositional distributions of STs are concerned, AD brains were similar to those in control brains. In conclusion, we suggest that MALDI-IMS is a useful tool for analysis of the distributions of various STs and this application might provide novel insight in the clinical study of demyelinating diseases.
D Yuki; Y Sugiura; N Zaima; H Akatsu; Y Hashizume; T Yamamoto; M Fujiwara; K Sugiyama; M Setou
Related Documents :
21262358 - Isolation and purification of a modified phenazine, griseoluteic acid, produced by stre...
21350658 - Improvement of physiological characteristic of selenium-enriched candida utilis with am...
16115608 - Regulation of cyclooxygenase catalysis by hydroperoxides.
9737538 - Thiyl radical-induced cis/trans-isomerization of methyl linoleate in methanol and of li...
8849848 - Does root surface conditioning with citric acid delay healing?
2562188 - The acidic amino-terminal region of the hiv-1 tat protein constitutes an essential acti...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-27
Journal Detail:
Title:  Neuroscience     Volume:  -     ISSN:  1873-7544     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-8-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Research and Development Headquarters, Lion Corporation, 7-2-1 Hirai, Edogawa-ku, Tokyo 132-0035, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Differential effects of histamine H(3) inverse agonist thioperamide, given alone or in combination w...
Next Document:  Potential mechanisms for hypoalgesia induced by anti-nerve growth factor immunoglobulin are identifi...