| Hydroxyl radical scavenging action of capsaicin. | |
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MedLine Citation:
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PMID: 12189045 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We recently reported that capsaicin (CAP) is capable of scavenging peroxyl radicals derived from 2,2'-azobis(2,4-dimethylvaleronitrile) as measured by electron spin resonance (ESR) spectroscopy. The present study describes the hydroxyl radical (HO*) scavenging ability of CAP as measured by DNA strand scission assay and by an ESR spin trapping technique with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The Fenton reaction [Fe(II)+ H(2)O(2) --> Fe(III) + HO* + HO(-)] was used as a source of HO*. The incubation of DNA with a mixture of FeSO(4) and H(2)O(2) caused DNA strand scission. The addition of CAP to the incubation mixture decreased the strand scission in a concentration-dependent manner. To understand the antioxidative mechanism of CAP, we used an ESR spin trapping technique. Kinetic competition studies using different concentrations of DMPO indicated that the decrease of the oxidative DNA damage was mainly due to the scavenging of HO* by CAP, not to the inhibition of the HO* generation system itself. We estimated the second order rate constants in the reaction of CAP and common HO* scavengers with HO* by kinetic competition studies. By comparison with the common HO* scavengers, CAP was found to scavenge HO* more effectively than mannitol, deoxyribose and ethanol, and to be equivalent to DMSO and benzoic acid, demonstrating that CAP is a potent HO* scavenger. The results suggest that CAP may act as an effective HO* scavenger as well as a peroxyl radical scavenger in biological systems. |
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Authors:
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Y Okada; H Okajima; Y Shima; H Ohta |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Redox report : communications in free radical research Volume: 7 ISSN: 1351-0002 ISO Abbreviation: Redox Rep. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-08-21 Completed Date: 2003-02-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9511366 Medline TA: Redox Rep Country: England |
Other Details:
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Languages: eng Pagination: 153-7 Citation Subset: IM |
Affiliation:
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Department of Analytical Chemistry, School of Health Sciences, Kyorin University, Miyashita-cho, Hachioji, Tokyo, 192-8508, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Capsaicin
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pharmacology* Cyclic N-Oxides / pharmacology DNA Damage Electron Spin Resonance Spectroscopy / methods Free Radical Scavengers / pharmacology* Hydroxyl Radical / metabolism* Spin Labels |
| Chemical | |
Reg. No./Substance:
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0/Cyclic N-Oxides; 0/Free Radical Scavengers; 0/Spin Labels; 3317-61-1/5,5-dimethyl-1-pyrroline-1-oxide; 3352-57-6/Hydroxyl Radical; 404-86-4/Capsaicin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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