Document Detail

Hydroxyl radical-induced strand break formation in single-stranded polynucleotides and single-stranded DNA in aqueous solution as measured by light scattering and by conductivity.
MedLine Citation:
PMID:  2902167     Owner:  NLM     Status:  MEDLINE    
Combining conductivity measurements and molecular weight determination by means of low-angle laser light scattering, we have found for the polyribonucleotides (polyuridylic acid (poly(U], polyadenylic acid (poly(A], polycytidylic acid (poly(C] and polyguanylic acid (poly(G] and for single-stranded DNA (ssDNA) that, on average, 8.5 counterions per single-strand break (ssb) are liberated under salt-free conditions. This relationship allows us to estimate, from conductivity measurements alone, G-values of single-strand break formation (G(ssb] for the polydeoxyribonucleotides (polydeoxyriboadenylic acid (poly(dA], polydeoxyribocytidylic acid (poly(dC], polydeoxyribothymidylic acid (poly(dT], polydeoxyribouridylic acid (poly(dU] and polydeoxyriboguanylic acid (poly(dG]. The following G(ssb) values (units of mumol J-1) have been obtained for anoxic conditions: poly(dA), 0.23; poly(dC), 0.14; poly(dT), 0.06; poly(dU), 0.046 and poly(dG), 0.009. Time-resolved conductivity measurements in pulse radiolysis enable us to measure the rate of strand break formation. The rate has been found to be similar for poly(dA) and ssDNA over a range of pH values. Poly(dC) and poly(dU) exhibit conductivity increase components with half-lives similar to those of poly(dA) and ssDNA at corresponding pH values. The implications of these results are discussed.
M Adinarayana; E Bothe; D Schulte-Frohlinde
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of radiation biology     Volume:  54     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-11-21     Completed Date:  1988-11-21     Revised Date:  2001-11-13    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  723-37     Citation Subset:  IM; S    
Max-Planck-Institut für Strahlenchemie, Mulheim a.d. Ruhr, F.R. Germany.
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MeSH Terms
DNA Damage*
DNA, Single-Stranded / drug effects*
Electric Conductivity
Free Radicals
Hydroxides / pharmacology*
Hydroxyl Radical
Scattering, Radiation
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Free Radicals; 0/Hydroxides; 0/Polyribonucleotides; 0/Solutions; 3352-57-6/Hydroxyl Radical; 7732-18-5/Water

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