Document Detail

Hydroxyl Radical (·OH) Played a Pivotal Role in Oridonin-Induced Apoptosis and Autophagy in Human Epidermoid Carcinoma A431 Cells.
MedLine Citation:
PMID:  23207767     Owner:  NLM     Status:  In-Data-Review    
Oridonin, a diterpenoid compound extracted and purified from Rabdosia rubescen, has been reported to induce tumor cell apoptosis through tyrosine kinase pathway. To further examine the mechanism of oridonin, we selected human epidermoid carcinoma A431 cell as a test object. Besides apoptosis, oridonin also induced A431 cell autophagy, and this autophagy antagonized apoptosis and played a protective role for A431 cells. Reactive oxygen species (ROS) played a pivotal role in induction of cytotoxicity. Therefore, a ROS scavenger, N-acetylcysteine (NAC) combined with oridonin was appiled. Results of morphologic observation, flow cytometric analysis and Western blot analysis showed that NAC could significantly reverse both ROS generation and down-regulation of mitochondrial membrane potential in oridonin treated cells. NAC inhibited oridonin induced apoptosis through both the intrinsic and extrinsic apoptotic pathways. NAC effectively inhibited both oridonin-induced apoptosis and autophagy by reducing intracellular oxidative stress. To further examine the mechanism of ROS, exogenous enzyme antioxidants (superoxide dismutase (SOD), catalase (CAT)) and non-enzyme antioxidants (glutathione (GSH)) were applied to detect the effect of oridonin on ROS generation. Only GSH exerted a similar role with NAC, suggesting that hydroxyl radical (·OH) played the major role in oridonin-induced cell death. Oridonin could decrease the GSH level in A431 cells in a dose-dependent manner. In addition, after treatment with ·OH donor, Fenton reagent, the changes in A431cells were similar to the results of oridonin treatment. All the results proved that ·OH played the pivotal role in oridonin induced apoptosis and autophagy in A431 cells.
Yang Yu; Si Miao Fan; Jun Ke Song; Shin-Ichi Tashiro; Satoshi Onodera; Takashi Ikejima
Related Documents :
25084177 - Gut commensalism, cytokines, and central nervous system demyelination.
24072877 - Inhibition of caspase-8 activity promotes protective th1- and th2-mediated immunity to ...
23737137 - Matrix metalloproteinase-2 (mmp-2) generates soluble hla-g1 by cell surface proteolytic...
23928767 - Microbial metabolites and senescence cytokines promote obesity-induced hcc.
12413067 - Osteoporosis in the rheumatic disease patient.
25015957 - Tlr4 regulates ifn-γ and il-17 production by both thymic and induced foxp3+ tregs duri...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  35     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2148-59     Citation Subset:  IM    
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Ameliorative effects of telmisartan on the inflammatory response and impaired spatial memory in a ra...
Next Document:  Effects of clioquinol analogues on the hypoxia-inducible factor pathway and intracelullar mobilizati...