| Hydroxyapatite-pulp composite fiber sheet bed: A new material for the immobilization of CHO-K1 cells. | |
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MedLine Citation:
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PMID: 19003282 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A new immobilization material for cell culture, ahydroxyapatite-pulp composite fiber (HAPC) sheet bed, was usedto grow CHO-K1 cells. The sheet bed for cell culture wasprepared from HAPC fiber by paper-making techniques. Scanning electron microscopic analysis revealed that the HAPCsheet bed had a structure consisting of piled fibers with spaces 10-200 mum in diameter and a pore surface area of 0.32 m(2) g(-1). Using a 25 x 25 mm(2) squareHAPC sheet bed 0.41 mm in thickness (85 g m(-2) basis weight) for cell culture, CHO-K1 cells grew to a cell densityof 3.7 x 10(7) cells cm(-3) in a 60 mm plastic dish over a 6-day culture period. High-density culture of CHO-K1 cells was successfully performed using the HAPC sheet bed in a 500 ml spinner flask over a 21-day culture period. The HAPC sheet bed, wound around the stirrer paddle, was rotated in the spinner flask in order to supply nutrientsand remove waste products efficiently. The HAPC sheet bedhas a large surface area to support cell growth and there islarge diffusion space inside of the bed. This newautoclavable substrate for anchorage-dependent cells can be easily scaled-up. |
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Authors:
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H Kawakatsu; S Ide; Y Okuda; S Shirahata |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 35 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2008-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 65-72 Citation Subset: - |
Affiliation:
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Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume-shi, Fukuoka, 839-0861, Japan. |
Export Citation:
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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