Document Detail

Hydrothermal production and characterization of protein and amino acids from silk waste.
MedLine Citation:
PMID:  18321700     Owner:  NLM     Status:  MEDLINE    
Non-catalytic hydrothermal decomposition of sericin and fibroin from silk waste into useful protein and amino acids was examined in a closed batch reactor at various temperatures, reaction times, and silk to water ratios to examine their effects on protein and amino acid yields. For the decomposition of sericin, the highest protein yield was found to be 0.466 mg protein/mg raw silk, obtained after 10 min hydrothermal reaction of silk waste at 1:100 silk to water ratio at 120 degrees C. The highest amino acid yield was found to be 0.203 mg amino acids/mg raw silk, obtained after 60 min of hydrothermal reaction of silk waste at 1:20 silk to water ratio at 160 degrees C. For the hydrothermal decomposition of fibroin, the highest protein yield was 0.455 mg protein/mg silk fibroin (1:100, 220 degrees C, 10 min) and that of amino acids was 0.755 mg amino acids/mg silk fibroin (1:50, 220 degrees C, 60 min). The rate of silk fibroin decomposition could be described by surface reaction kinetics. The soluble reaction products were freeze-dried to obtain sericin and fibroin particles, whose conformation and crystal structure of the particles were shown to differ from the original silk materials, particularly in the case of fibroin, in which the change from beta-sheet conformation to alpha-helix/random coil was observed.
Wiwat Lamoolphak; Wanchai De-Eknamkul; Artiwan Shotipruk
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-05
Journal Detail:
Title:  Bioresource technology     Volume:  99     ISSN:  0960-8524     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-06-23     Completed Date:  2008-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  7678-85     Citation Subset:  IM    
Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Patumwan, Phayathai Road, Bangkok 10330, Thailand.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / biosynthesis*
Calorimetry, Differential Scanning
Fibroins / chemistry,  metabolism,  ultrastructure
Freeze Drying
Industrial Waste
Molecular Weight
Particle Size
Protein Conformation
Proteins / metabolism*
Sericins / chemistry,  metabolism,  ultrastructure
Spectroscopy, Fourier Transform Infrared
Time Factors
Water / chemistry
X-Ray Diffraction
Reg. No./Substance:
0/Amino Acids; 0/Industrial Waste; 0/Proteins; 0/Sericins; 0/Silk; 7732-18-5/Water; 9007-76-5/Fibroins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Start-up of an UASB-septic tank for community on-site treatment of strong domestic sewage.
Next Document:  Glycosyl and polyalcoholic prodrugs of lonidamine.