Document Detail


Hydrostatic pressure studies of native and synthetic thick filaments: in vitro myosin aggregates at pH 7.0 with and without C-protein.
MedLine Citation:
PMID:  2574997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Column-purified myosin at pH 7.0 will reproducibly aggregate into filaments of known average length and structure when dialyzed against a low ionic strength medium under controlled conditions. When exposed to increased hydrostatic pressure, followed by quick return to atmospheric pressure, the original filaments shorten linearly with increasing pressure; in addition, a second population of filaments is seen, presumably the result of reaggregation of myosin after release of pressure. This second population is about 0.5 microns long, bipolar, and about half the diameter of the original filaments. The number of these filaments, but not their physical characteristics, is a function of the shortening of the original filament population. Both the remnants of the original population and the new aggregates, once formed, are stable over time and at room temperature. The addition of C-protein to myosin solutions before filament preparation results in a filament population of slightly shorter length. When these filaments are exposed to increased hydrostatic pressure, they are more resistant to disaggregation than myosin filaments without C-protein. However, like the filaments prepared in the absence of C-protein, a second population of shorter, thinner filaments is visible after exposure to pressure.
Authors:
S J Tumminia; J F Koretz; J V Landau
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  999     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-02-13     Completed Date:  1990-02-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  300-12     Citation Subset:  IM    
Affiliation:
Center for Biophysics, Rensselaer Polytechnic Institute, Troy, NY 12180-3590.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ca(2+) Mg(2+)-ATPase / analysis
Carrier Proteins*
Dialysis
Hydrogen-Ion Concentration
Hydrostatic Pressure*
Microscopy, Electron
Muscles / analysis*,  ultrastructure
Myosins / analysis*,  ultrastructure
Nephelometry and Turbidimetry
Pressure*
Rabbits
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/citrate-binding transport protein; EC 3.6.1.-/Ca(2+) Mg(2+)-ATPase; EC 3.6.4.1/Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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