Document Detail


Hydrostatic Pressure Effects on the Fluorescence and FRET Behavior of Cy3-labeled Phycocyanin System.
MedLine Citation:
PMID:  22917470     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
FRET has been used as a powerful tool in biological fields as biosensors, bioimaging, protein folding/unfolding monitoring, biomolecular interactions and so on. It is also important to applying FRET to high hydrostatic pressure studies on bio- or bio-related systems. Herein, we construct a FRET system by labeling Cy3 on C-phycocyanin (C-PC) to investigate the effect of hydrostatic pressure on the fluorescence and FRET behavior between them. The fluorescence spectra of individual Cy3, C-PC and integrated Cy3/C-PC system are measured separately under compression. An enhanced FRET efficiency under compression is concluded based on fluorescence behavior differences between them. In order to further reveal the origination of the enhanced FRET efficiency with pressure, the overlap integral between Cy3 emission and C-PC absorption is also calculated, and several possible explanations are proposed.
Authors:
Yong-Qiang Dang; Qian Li; Kai Wang; Yuqing Wu; Lili Lian; Bo Zou
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-23
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  -     ISSN:  1520-5207     ISO Abbreviation:  J Phys Chem B     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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