Document Detail


Hydrophobic membrane thickness and lipid-protein interactions of the leucine transport system of Lactococcus lactis.
MedLine Citation:
PMID:  1905573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of the phospholipid acyl chain carbon number on the activity of the branched-chain amino acid transport system of Lactococcus lactis has been investigated. Major fatty acids identified in a total lipid extract of L. lactis membranes are palmitic acid (16:0), oleic acid (18:1) and the cyclopropane-ring containing lactobacillic acid (19 delta). L. lactis membrane vesicles were fused with liposomes prepared from equimolar mixtures of synthetic phosphatidylethanolamine (PE) and phosphatidylcholine (PC) with cis mono-unsaturated acyl chains. The activity of the branched-chain amino acid carrier is determined by the bulk properties of the membrane (Driessen, A.J.M., Zheng, T., In 't Veld, G., Op den Kamp, J.A.F. and Konings, W.N. (1988) Biochemistry 27, 865-872). PE acts as an activator and PC is ineffective. Counterflow and protonmotive-force driven transport of leucine is sensitive to changes in the acyl chain carbon number of both phospholipids and maximal with dioleoyl-PE/dioleoyl-PC. Above the gel to liquid-crystalline phase transition temperature of the lipid species, membrane fluidity decreased with increasing acyl chain carbon number. Our data suggest that the carbon number of the acyl chains of PE and PC determine to a large extent the activity of the transport system. This might be relevant for the interaction of PE with the transport protein. Variations in the acyl chain composition of PC exert a more general effect on transport activity. The acyl chain composition of phospholipids determines the membrane thickness (Lewis, B.A. and Engelman, D.M. (1983) J. Mol. Biol. 166, 211-217). We therefore propose that the degree of matching between the lipid-bilayer and the hydrophobic thickness of the branched-chain amino acid carrier is an important parameter in lipid-protein interactions.
Authors:
G In 't Veld; A J Driessen; J A Op den Kamp; W N Konings
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1065     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-08-08     Completed Date:  1991-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  203-12     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Groningen, Haren, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Cell Membrane / chemistry,  metabolism,  ultrastructure*
Cell Membrane Permeability
Diphenylhexatriene / analogs & derivatives
Fatty Acids / analysis
Fluorescence Polarization
Fluorescent Dyes
Freeze Fracturing
Kinetics
Lactococcus lactis / metabolism*,  ultrastructure
Leucine / metabolism*
Liposomes / metabolism
Membrane Fluidity
Microscopy, Electron
Oleic Acid
Oleic Acids / analysis
Palmitic Acid
Palmitic Acids / analysis
Phosphatidylcholines / analysis,  metabolism
Phosphatidylethanolamines / analysis,  metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fluorescent Dyes; 0/Liposomes; 0/Oleic Acids; 0/Palmitic Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 112-80-1/Oleic Acid; 1720-32-7/Diphenylhexatriene; 503-06-0/phytomonic acid; 57-10-3/Palmitic Acid; 61-90-5/Leucine; 71316-28-4/1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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