| Hydrophilic polyester microspheres: effect of molecular weight and copolymer composition on release of BSA. | |
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MedLine Citation:
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PMID: 20602152 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To study the release of a model protein, bovine serum albumin (BSA), from microspheres of an hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (PLHMGA). METHODS: BSA-loaded microspheres were prepared by a double emulsion solvent evaporation method. The effect of copolymer composition and the molecular weight of the copolymer on in vitro release and degradation were studied. The integrity of the released BSA was studied by fluorescence spectroscopy and size exclusion chromatography (SEC). RESULTS: Microspheres prepared from PLHMGA with 50% hydroxymethyl glycolic acid (HMG) showed a burst release followed by a sustained release in 5-10 days. PLHMGA microspheres prepared from a copolymer with 35% and 25% HMG showed a sustained release of BSA up to 80% for 30 and 60 days, respectively. The release of BSA was hardly affected by the molecular weight of the polymer. Fluorescence spectroscopy and SEC showed that the released BSA preserved its structural integrity. Microspheres were fully degradable, and the degradation time increased from approximately 20 days to 60 days when the HMG content decreased from 50% to 25%. CONCLUSIONS: Taking the degradation and release data together, it can be concluded that the release of BSA from PLHMGA microspheres is governed by degradation of the microspheres. |
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Authors:
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Amir H Ghassemi; Mies J van Steenbergen; Herre Talsma; Cornelus F van Nostrum; Daan J A Crommelin; Wim E Hennink |
Publication Detail:
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Type: Journal Article Date: 2010-07-03 |
Journal Detail:
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Title: Pharmaceutical research Volume: 27 ISSN: 1573-904X ISO Abbreviation: Pharm. Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-05 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406521 Medline TA: Pharm Res Country: United States |
Other Details:
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Languages: eng Pagination: 2008-17 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB, Utrecht, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel Drug Carriers / chemistry* Drug Compounding Hydroxylation Microspheres Molecular Structure Molecular Weight Particle Size Polyesters / chemistry* Protein Stability Serum Albumin, Bovine / chemistry* Solubility Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Polyesters; 0/Serum Albumin, Bovine; 0/poly(lactic-co-hydroxymethyl glycolic acid) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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