Document Detail


Hydroperoxidase I catalyzes peroxidative activation of 3,3'-dichlorobenzidine to a mutagen in Salmonella typhimurium.
MedLine Citation:
PMID:  2241156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dichlorobenzidine can be peroxidatively activated in Salmonella typhimurium Ames tester strains. Mutagenicity is observed when an S. typhimurium strain which is sensitive to frame-shift mutagens is incubated with dichlorobenzidine and hydrogen peroxide. In this paper, we show that the bacterial enzyme, hydroperoxidase I, is responsible for much of this activation. We constructed isogenic tester strains which lack hydroperoxidase I or II, due to Tn10 insertions in the structural genes encoding these proteins. Hydrogen peroxide-dependent mutagenicity of dichlorobenzidine was measured in each strain. A tester strain lacking hydroperoxidase I activity was much less sensitive than was the parent strain. When hydroperoxidase I activity was restored in this strain, via a plasmid-borne copy of the gene encoding the Escherichia coli protein, sensitivity to peroxide-dependent dichlorobenzidine mutagenicity was enhanced.
Authors:
I J McGowan-Jordan; P D Josephy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  282     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-04     Completed Date:  1990-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  352-7     Citation Subset:  IM    
Affiliation:
Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Guelph, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
3,3'-Dichlorobenzidine / pharmacokinetics*,  toxicity
Benzidines / pharmacology
Biotransformation
Catalase / metabolism*
Escherichia coli Proteins*
Fluorenes / pharmacology
Hydrogen Peroxide / toxicity*
Mutagenicity Tests
Mutagens*
Mutation
Oxidation-Reduction
Salmonella typhimurium / enzymology*,  genetics,  metabolism
Chemical
Reg. No./Substance:
0/Benzidines; 0/Escherichia coli Proteins; 0/Fluorenes; 0/Mutagens; 153-78-6/2-aminofluorene; 54827-17-7/3,3',5,5'-tetramethylbenzidine; 7722-84-1/Hydrogen Peroxide; 91-94-1/3,3'-Dichlorobenzidine; EC 1.11.1.6/Catalase; EC 1.11.1.6/katG protein, E coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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