| Hydrolysis under high hydrostatic pressure as a means to reduce the binding of beta-lactoglobulin to immunoglobulin E from human sera. | |
| | |
MedLine Citation:
|
PMID: 18680946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cows' milk allergy is the most frequent food allergy in children, and beta-lactoglobulin (beta-Lg) is a major allergen. Milk-based hypoallergenic ingredients are manufactured by enzymatic hydrolysis, a process that could be improved by the application of high-pressure treatments. This study showed that the treatment of beta-Lg dissolved in buffer with chymotrypsin and trypsin under high pressure for relatively short times accelerated proteolysis by leading to a rapid removal of the intact protein. The rapid proteolysis of the beta-Lg substrate under pressure led to the production, in 20 min, of hydrolysates with lower immunoglobulin (Ig) G binding than those produced in 8 h (chymotrypsin) or 48 h (trypsin) at atmospheric pressure. However, those hydrolysates retained some residual IgE-binding properties that could be traced to the preferential release, during the initial stages of proteolysis, of peptides containing IgE epitopes, such as (Val-41-Lys-60), (Leu-149-Ile-162), and (Ser-21-Arg-40). The formation of these fragments was favored when proteolysis was conducted under high pressure due to the preferential hydrolysis of Arg-40 and Arg-148 by trypsin, and Tyr-42 and Leu-149 by chymotrypsin, all located at the dimer interface of beta-Lg or very close to it. Although our results do not support that trypsin and chymotrypsin under high pressure selectively address the allergenic regions of beta-Lg, it is possible to select the conditions that quickly produce hydrolysates with reduced potential allergenicity that could be used in hypoallergenic foods. |
| | |
Authors:
|
R Chicón; J Belloque; E Alonso; P J Martín-Alvarez; R López-Fandiño |
Related Documents
:
|
2726286 - Cardiovascular effects induced by progesterone administration. 2882016 - Systemic and regional hemodynamic characterization of alpha-1 and alpha-2 adrenoceptor ... 22645856 - Hemodynamic assessment of mitral stenosis: mitral valve resistance as an echocardiograp... 1968346 - The effect of autonomic blockers on heart rate: a comparison between two ethnic groups. 8874296 - Starling resistor effects on pulmonary artery occlusion pressure in endotoxin shock pro... 8347316 - Spectral analysis of 24 h blood pressure recordings. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of food protection Volume: 71 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2008 Jul |
Date Detail:
|
Created Date: 2008-08-06 Completed Date: 2008-09-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
|
Languages: eng Pagination: 1453-9 Citation Subset: IM |
Affiliation:
|
Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, High Pressure Liquid Chymotrypsin / metabolism Epitopes Hydrolysis Hydrostatic Pressure* Immunoglobulin E / immunology, metabolism* Lactoglobulins / immunology, metabolism* Milk / enzymology*, metabolism Milk Hypersensitivity / immunology, prevention & control Peptide Fragments Time Factors Trypsin / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Epitopes; 0/Lactoglobulins; 0/Peptide Fragments; 37341-29-0/Immunoglobulin E; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A qualitative assessment of Toxoplasma gondii risk in ready-to-eat smallgoods processing.
Next Document: Salmonella prevalence in seafood imported into Japan.