| Hydrolysis of surfactant-associated phosphatidylcholine by mammalian secretory phospholipases A2. | |
| | |
MedLine Citation:
|
PMID: 9755106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Hydrolysis of surfactant-associated phospholipids by secretory phospholipases A2 is an important potential mechanism for surfactant dysfunction in inflammatory lung diseases. In these conditions, airway secretory phospholipase A2 (sPLA2) activity is increased, but the type of sPLA2 and its impact on surfactant function are not well understood. We examined in vitro the effect of multiple secretory phospholipases A2 on surfactant, including their ability to 1) release free fatty acids, 2) release lysophospholipids, and 3) increase the minimum surface tension (gammamin) on a pulsating bubble surfactometer. Natural porcine surfactant and Survanta were exposed to mammalian group I (recombinant porcine pancreatic) and group II (recombinant human) secretory phospholipases A2. Our results demonstrate that mammalian group I sPLA2 hydrolyzes phosphatidylcholine (PC), producing free fatty acids and lysophosphatidylcholine, and increases gammamin. In contrast, mammalian group II sPLA2 demonstrates limited hydrolysis of PC and does not increase gammamin. Group I and group II secretory phospholipases A2 from snake venom hydrolyze PC and inhibit surfactant function. In summary, mammalian secretory phospholipases A2 from groups I and II differ significantly from each other and from snake venom in their ability to hydrolyze surfactant-associated PC. |
| | |
Authors:
|
R D Hite; M C Seeds; R B Jacinto; R Balasubramanian; M Waite; D Bass |
Related Documents
:
|
8389716 - Behavioural and biochemical effects of acute central metabolic inhibition: effects of a... 168186 - Postitional specificity of fatty acids in pyrophosphatidic acid from cryptococcus neofo... 8944746 - Bezafibrate and clofibric acid are novel inhibitors of phosphatidylcholine synthesis vi... 3841576 - Pulmonary surfactant lipid synthesis from ketone bodies, lactate and glucose in newborn... 8651166 - Effect of rennie liquid versus maalox liquid on intragastric ph in a double-blind, rand... 18803456 - Azelaic acid 15% gel in the treatment of rosacea. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American journal of physiology Volume: 275 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1998 Oct |
Date Detail:
|
Created Date: 1998-11-23 Completed Date: 1998-11-23 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: L740-7 Citation Subset: IM |
Affiliation:
|
Section on Pulmonary and Critical Care, Department of Internal Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Products* COS Cells Fatty Acids, Nonesterified / metabolism Group II Phospholipases A2 Humans Hydrolysis Lung / enzymology Lysophospholipids / metabolism Mammals Pancreas / enzymology Phosphatidylcholines / metabolism* Phospholipases A / metabolism* Phospholipases A2 Pulmonary Surfactants / chemistry*, metabolism* Recombinant Proteins / metabolism Snake Venoms Substrate Specificity Surface Tension Swine Transfection |
| Grant Support | |
ID/Acronym/Agency:
|
CA-12107/CA/NCI NIH HHS; P01-HL-50395/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Biological Products; 0/Fatty Acids, Nonesterified; 0/Lysophospholipids; 0/Phosphatidylcholines; 0/Pulmonary Surfactants; 0/Recombinant Proteins; 0/Snake Venoms; 108778-82-1/beractant; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Group II Phospholipases A2; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ET-1 modulates KCa-channel activity and arterial tension in normoxic and hypoxic human pulmonary vas...
Next Document: Role of G proteins in agonist-induced Ca2+ sensitization of tracheal smooth muscle.