| Hydrolysis of short acyl chain inositol lipids by phospholipase C-delta 1. | |
| | |
MedLine Citation:
|
PMID: 8380575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We investigated the relationship between substrate aggregation and activation of phosphoinositide-specific phospholipase C-delta 1 (PLC-delta 1), isolated from bovine brain cytosol. The inositol lipids 1,2-dibutyryl-sn-glycero-3-phosphoinositol (di-C4-PI), 1,2-dihexanoyl-sn-glycero-3-phosphoinositol (di-C6-PI), and 1,2-dioctanoyl-sn-glycero-3-phosphoinositol (di-C8-PI) were prepared from synthetic cytidine diphosphate diglyceride analogs in a reaction with myo-inositol catalyzed by yeast phosphatidylinositol synthase. All three lipids served as substrates for PLC-delta 1 at concentrations significantly below their critical micelle concentration (cmc). Under these conditions, steps that might limit the reaction rate, such as membrane adsorption or penetration into the phospholipid surface, were eliminated. Below the cmc, the concentration of lipid substrate required to produce hydrolysis followed the order: di-C8-PI < di-C6-PI << di-C4-PI. Calcium was essential for hydrolysis of the short chain substrates at all lipid concentrations tested. The dependence of the reaction on calcium suggests that this ion activates PLC-delta 1 at a step other than adsorption to or penetration of the membrane surface. As the concentration of di-C8-PI was raised above the cmc, the reaction velocity increased 2-3-fold. These results are consistent with the idea that micellar or bilayer aggregates of phosphoinositol are not required for PLC-catalyzed hydrolysis, although the reaction rate is enhanced by micelle formation. |
| | |
Authors:
|
M J Rebecchi; R Eberhardt; T Delaney; S Ali; R Bittman |
Related Documents
:
|
15209095 - Sucrose transport into plasma membrane vesicles from tobacco leaves by h+ symport or co... 4052575 - Thermodynamics of the conversion of fumarate to l-(-)-malate. 14741585 - Quantification of photosystem i and ii in different parts of the thylakoid membrane fro... 9159645 - Temperature dependence of the inward rectifier k+ channel gating in guinea-pig ventricu... 3509705 - Assessment of two alternative methods for predicting the in vivo toxicities of metallic... 1492585 - The value of an electronic microcomputerized basal body temperature measurement device ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 268 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1993 Jan |
Date Detail:
|
Created Date: 1993-02-18 Completed Date: 1993-02-18 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1735-41 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Biophysics, State University of New York, Stony Brook 11794. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / enzymology CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase Calcium / pharmacology Cattle Cytosol / enzymology Enzyme Activation / drug effects Hydrolysis Kinetics Phosphatidylinositols / metabolism* Phosphotransferases / metabolism Saccharomyces cerevisiae / enzymology Substrate Specificity Transferases (Other Substituted Phosphate Groups)* Type C Phospholipases / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
GM-43422/GM/NIGMS NIH HHS; HL-16660/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Phosphatidylinositols; 105172-95-0/1,2-dioctanoyl-sn-glycero-3-phosphoinositol; 148437-41-6/1,2-dihexanoyl-sn-glycero-3-phosphoinositol; 148504-92-1/1,2-dibutyryl-sn-glycero-3-phosphoinositol; 7440-70-2/Calcium; EC 2.7.-/Phosphotransferases; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 2.7.8.11/CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of intracellular amino acid concentrations, cyclic AMP, and dexamethasone on lysosomal prote...
Next Document: Cumulative effect of double-site mutations of human epidermal growth factor on receptor binding.