Document Detail

Hydrolysis of phosphatidylinositol by human endometrium: modulating effects of steroids on arachidonic acid and 1,2-diacylglycerol release.
MedLine Citation:
PMID:  3379359     Owner:  NLM     Status:  MEDLINE    
Phospholipase C and 1,2-diacylglycerol lipase activities were demonstrated in human endometrium using 1-stearoyl-2-[1-14C]arachidonyl phosphatidylinositol as substrate. Phosphatidylinositol is hydrolysed by phospholipase C to inositol phosphates and to 1,2-diacylglycerol which is then further metabolized by 1,2-diacylglycerol lipase to release free arachidonic acid. In the present study the radiolabelled products formed (1,2-diacylglycerol and arachidonic acid) were measured following chloroform/methanol extraction and thin-layer chromatography. Phospholipase C activity was calcium dependent and optimal at pH 5.0-5.5 and 7.5; 1,2-diacylglycerol lipase activity was also calcium dependent, with an optimum pH of 5.5. A significant increase in 1,2-diacylglycerol production was stimulated by steroid sulphates. Pregnenolone sulphate, oestrone sulphate, testosterone sulphate and dehydroepiandrosterone sulphate stimulated 4, 3.2-, 1.8- and 2.6-fold increases in release respectively. Oestradiol sulphate stimulated a 25% increase in diacylglycerol release which was not significantly different from the control value. Progesterone stimulated a fourfold increase but other free steroids had no effect. Arachidonic acid release was increased in the presence of oestradiol sulphate, oestrone and oestradiol but reduced by oestrone sulphate, dehydroepiandrosterone sulphate, progesterone, dehydroepiandrosterone and, to a lesser extent, by pregnenolone sulphate and testosterone sulphate. 5-Androstene-3 beta,17 beta-diol had no effect on the liberation of either product. This study demonstrates a potential route for the liberation of arachidonic acid from phosphatidylinositol in human endometrium. The opposing effects of steroids on phospholipase C and 1,2-diacylglycerol lipase activity could be important in regulating the release of arachidonic acid by this pathway.
R C Bonney; S Franks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  117     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-07-25     Completed Date:  1988-07-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  309-14     Citation Subset:  IM    
Department of Chemical Pathology, St Mary's Hospital Medical School, London.
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MeSH Terms
Arachidonic Acids / metabolism*
Calcium / metabolism
Diglycerides / metabolism
Endometrium / drug effects,  enzymology*
Gonadal Steroid Hormones / pharmacology*
Lipoprotein Lipase / metabolism*
Phosphatidylinositols / metabolism
Pregnenolone / pharmacology
Type C Phospholipases / metabolism*
Reg. No./Substance:
0/1,2-diacylglycerol; 0/Arachidonic Acids; 0/Diglycerides; 0/Gonadal Steroid Hormones; 0/Phosphatidylinositols; 145-13-1/Pregnenolone; 7440-70-2/Calcium; EC Lipase; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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