Document Detail

Hydrolysis of nuclear phospholipids in relation with proliferative state in uterine stromal cells.
MedLine Citation:
PMID:  15194229     Owner:  NLM     Status:  MEDLINE    
The current study examined the metabolism of phospholipid (PL) in the whole cell homogenate and in the nuclear fraction in proliferative and non-proliferative uterine stromal cells (U(III) cells). Growth arrested cells were obtained either from contact-inhibited confluent cells or from proliferative cells treated with aristolochic acid (AR) for 2 days. Fatty acid composition and fatty acid amount of both total and nuclear PL were not significantly different between proliferative, confluent and AR-treated cells. In contrast, marked differences were observed in the incorporation of [(3)H]AA, with greater incorporation in proliferative cells than in confluent or AR-treated cells, particularly in nuclear PL. Considering endogenous level of arachidonic acid (AA) in total and nuclear PL, we found that AA turnover in nuclear PL was especially high compared to that in total PL and that this difference was accentuated in proliferative cells compared to non-proliferative cells. Interestingly, [(3)H]AA incorporation and AA turnover in proliferative, confluent and AR-treated cells vary accordingly to the expression, activity and/or content of pancreatic phospholipase A(2) (PLA(2)-I) in the nuclear compartment of these cells that we reported in previous studies. The changes in metabolism of nuclear PL during cell proliferation are consistent with an enhanced PL hydrolysis that could involve PLA(2)-I.
Isabelle Delton-Vandenbroucke; Patricia Lemaire; Michel Lagarde; Christian Laugier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  86     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:    2004 Apr-May
Date Detail:
Created Date:  2004-06-14     Completed Date:  2005-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
UMR 585 Inserm, INSA Lyon, 69621 Villeurbanne cedex, France.
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MeSH Terms
Arachidonic Acid / pharmacology
Aristolochic Acids / pharmacology
Carcinogens / pharmacology
Cell Nucleus / metabolism*
Cell Proliferation*
Pancreas / enzymology
Phospholipases A / metabolism
Phospholipids / metabolism*
Stromal Cells / metabolism*
Uterus / metabolism*
Reg. No./Substance:
0/Aristolochic Acids; 0/Carcinogens; 0/Phospholipids; 313-67-7/aristolochic acid I; 506-32-1/Arachidonic Acid; EC 3.1.1.-/Phospholipases A

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