| Hydrolysis of menhaden oil by a Candida cylindracea lipase immobilized in a hollow-fiber reactor. | |
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MedLine Citation:
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PMID: 10099579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A lipase from Candida cylindracea immobilized by adsorption on microporous polypropylene fibers was used to selectively hydrolyze the saturated and monounsaturated fatty acid residues of menhaden oil at 40 degrees C and pH 7.0. At a space time of 3.5 h, the shell and tube reactor containing these hollow fibers gives a fractional release of each of the saturated and monounsaturated fatty acid residues (i.e., C14, C16, C16:1, C18:1) of ca. 88% of the corresponding possible asymptotic value. The corresponding coproduct glycerides retained over 90% of the initial residues of both eicosapentaenoic (EPA; C20:5) and docosahexaenoic (DHA; C22:6) acids. The half-life of the immobilized lipase was 170 h when the reactor was operated at the indicated (optimum) conditions. Rate expressions associated with a generic ping-pong bi-bi mechanism were used to fit the experimental data for the lipase catalyzed reaction. Both uni- and multiresponse nonlinear regression methods were employed to determine the kinetic parameters associated with these rate expressions. The best statistical fit of the uniresponse data was obtained for a rate expression, which is formally equivalent to a general Michaelis-Menten mechanism. After reparameterization, this rate expression reduced to a pseudo-first-order model. For the multiresponse analysis, a model that employed a normal distribution of the ratio of Vmax/Km with respect to the chain length of the fatty acid residues provided the best statistical fit of the experimental data. |
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Authors:
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K E Rice; J Watkins; C G Hill |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 63 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-04-26 Completed Date: 1999-04-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 33-45 Citation Subset: IM |
Copyright Information:
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Copyright 1999 John Wiley & Sons, Inc. |
Affiliation:
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Department of Chemical Engineering, University of Wisconsin, 1415 Engineering Drive, Madison, Wisconsin 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors Biotechnology / methods Candida / enzymology* Docosahexaenoic Acids / metabolism* Eicosapentaenoic Acid / metabolism* Enzymes, Immobilized / metabolism* Fish Oils / metabolism* Hydrogen-Ion Concentration Hydrolysis Kinetics Lipase / metabolism* Regression Analysis Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Fish Oils; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids; 8002-50-4/Menhaden oil; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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