| Hydrolysis of lactose in skim milk by immobilized beta-galactosidase in a spiral flow reactor. | |
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MedLine Citation:
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PMID: 18587857 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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beta-galactosidase from Aspergillus Oryzae immobilized in a spiral flow reactor was used to effect the hydrolysis of the lactose component of skim milk. Residence time distribution measurements were used to assess the amount of longitudinal dispersion occurring as a consequence of the spiral flow pattern and the semiporous nature of the polymeric material used to construct the spiral. It was possible to model the flow conditions as tubular flow with a Peclet number that was a linear function of the reactor space time. Nonlinear regression methods were used to determine the kinetic parameters of three proposed enzymatic rate expressions. The best fit of the data was obtained using a rate expression containing separate terms for competitive inhibition of the reaction by both the a and beta anomers of galactose. This kinetic model also incorporates the kinetics of the mutarotation between these forms. At 30 degrees C and a space time of 7 minutes, 80% of the lactose present in skim milk can be converted to glucose and galactose. |
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Authors:
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A P Bakken; C G Hill; C H Amundson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 33 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1989 Apr |
Date Detail:
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Created Date: 2008-06-30 Completed Date: 2009-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 1249-57 Citation Subset: - |
Affiliation:
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Department of Chemical Engineering and Food Science, University of Wisconsin, Madison, Wisconsin 53706, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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