| Hydrolysis of histones by proteinases. | |
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MedLine Citation:
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PMID: 2968788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydrolysis of histones by proteinases from rat liver, skin and other sources was studied by using a rat thymus histone preparation as the substrate and polyacrylamide-gel electrophoresis and densitometric analysis as the methods to detect histone subtypes and their hydrolysis. The rat mast-cell proteinase I effectively hydrolysed histones except type H4. Thrombin hydrolysed effectively histones H1 and H2A, whereas plasmin hydrolysed all types of histones. Cathepsin D hydrolysed especially histone H2A. Cathepsins B and L hydrolysed all histones more slowly, and cathepsin H hydrolysed them extremely slowly. Epidermal aminoendopeptidase did not hydrolyse histones. Trypsin and chymotrypsin were used as reference enzymes, which hydrolysed all types of histones in very low concentrations. This study suggests that a variety of proteinases could play a role in histone hydrolysis. Hydrolysis of a specific subtype of histones, such as histone H2A at pH 6 by cathepsin D, may be directly involved in regulation of epidermal-cell differentiation. |
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Authors:
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R J Harvima; K Yabe; J E Fräki; K Fukuyama; W L Epstein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 250 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-08-05 Completed Date: 1988-08-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 859-64 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University of Kuopio, Finland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminopeptidases
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metabolism Animals Cathepsins / metabolism Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Endopeptidases / metabolism* Epidermis / enzymology Fibrinolysin / metabolism Histones / metabolism* Hydrolysis Liver / enzymology Rats Rats, Inbred Strains Thrombin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AM12433/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Histones; EC 3.4.-/Cathepsins; EC 3.4.-/Endopeptidases; EC 3.4.11.-/Aminopeptidases; EC 3.4.21.5/Thrombin; EC 3.4.21.7/Fibrinolysin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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