Document Detail


Hydrolysis of cis- and trans-epoxyeicosatrienoic acids by rat red blood cells.
MedLine Citation:
PMID:  18445784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythrocytes serve as reservoirs for cis- and trans-epoxyeicosatrienoic acids (EETs). Incubation of rat red blood cells (RBCs) with cis- and trans-EETs produces threo- and erythro-dihydroxyeicosatrienoic acids, respectively. The V(max) of EET hydrolysis by rat intact RBCs (2.35 +/- 0.24 pmol/min/10(8) RBCs for 14,15-trans-EET) decreased by approximately 2 to 3-fold sequentially from 14,15-, 11,12- to 8,9-EETs for both cis- and trans-isomers. The V(max) of trans-EET hydrolysis by RBCs is approximately 2 to 3 times that of the corresponding cis-EETs. Incubation of EETs with recombinant murine soluble epoxide hydrolase (sEH) yielded the same geometric and regio preferences of EET hydrolysis as with rat intact RBCs. The principal epoxide hydrolase activity for EET hydrolysis (approximately 90%) is present in the erythrocyte cytosol. Western blots of sEH suggested a concentration of sEH protein to be approximately 2 microg/mg protein or 0.4 microg/10(9) RBCs. The apparent K(m) values of EETs were between 1 and 2 microM, close to the K(m) for purified sEH as reported. Erythrocyte hydration of cis- and trans-EETs was blocked by sEH inhibitors, 1,3-dicyclohexylurea and 4-[4-(3-adamantan-1-ylureido)cyclohexyloxy]benzoic acid. Erythrocyte sEH activity was inhibited more than 80% by 0.2% bovine serum albumin in the buffer. Preferred hydrolysis of 14,15-EETs and trans-epoxides characterizes sEH activity in RBCs that regulates the hydrolysis and release of cis- and trans-EETs in the circulation. Inhibition of sEH has produced antihypertensive and antiinflammatory effects. Because plasma trans-EETs would increase more than cis-EETs with sEH inhibition, the potential roles of trans-EETs and erythrocyte sEH in terms of circulatory regulation deserve attention.
Authors:
Houli Jiang; Angela G Zhu; Magdalena Mamczur; Christophe Morisseau; Bruce D Hammock; John R Falck; John C McGiff
Related Documents :
11093824 - Synergistic induction of apoptosis of neuroblastoma by fenretinide or cd437 in combinat...
8202504 - Limb and lower-body duplications induced by retinoic acid in mice.
16712844 - Cyp4a11 is repressed by retinoic acid in human liver cells.
3025324 - Effects of dietary retinoic acid on cellular retinol- and retinoic acid-binding protein...
17090114 - New polyphenol derivative in ipomoea batatas tubers and its antioxidant activity.
18618464 - Evaluation of protein, peptide, and amino acid retention on c5 hydride-based stationary...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-04-29
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  326     ISSN:  1521-0103     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-19     Completed Date:  2008-07-30     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  330-7     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, New York Medical College, Valhalla, NY 10595, USA. houli_jiang@nymc.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Erythrocytes / metabolism*
Hydrolysis
Hydroxyeicosatetraenoic Acids / blood*,  chemistry*
Male
Molecular Conformation
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
HL-25394/HL/NHLBI NIH HHS; P42 ES004699/ES/NIEHS NIH HHS; PPG 34300//PHS HHS; R01 HL025394-30/HL/NHLBI NIH HHS; R37 ES02710/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Hydroxyeicosatetraenoic Acids
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Age-dependent development of metabolic derangement and effects of intervention with pioglitazone in ...
Next Document:  The complex of TFII-I, PARP1, and SFPQ proteins regulates the DYX1C1 gene implicated in neuronal mig...