Document Detail


Hydrolysis of acyl-homogeneous and fish oil triacylglycerols using desalted midgut extract from atlantic salmon, Salmo salar.
MedLine Citation:
PMID:  18493809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite several studies aimed at evaluating the positional and fatty acid specificity of fish triacylglycerol (TAG) digestive lipases, there is still much uncertainty regarding these issues. The aim of the present study was therefore to address these questions in Atlantic salmon (Salmo salar L.). Crude luminal midgut extracts were collected from fed salmon and the hydrolysis studied for various substrates including triolein (Tri-18:1), trilinolein (Tri-18:2), trilinolenin (Tri-18:3), trieicosapentaenoin (Tri-20:5), tridocosahexaenoin (Tri-22:6) and natural fish oil TAG. Using Tri-18:1, in a time-curve model showed an initial high degree of sn-1 or sn-3 specificity where sn-1,2(2,3)-diacylglycerol (1,2(2,3)-DAG) and free fatty acid (FFA) were the main hydrolytic products up to 15 min. Lack of initial sn-2 specificity was confirmed by negligible sn-1,3-diacylglycerol (1,3-DAG) being produced. During the further hydrolysis of DAG, all positions appeared susceptible to attack causing a concomitantly small increase in sn-1(3)-monoacylglycerol (1(3)-MAG) and 2-MAG, but not at the level expected for an exclusively sn-1,3-specific lipase. Oleic acid (18:1n-9) and eicosapentaenoic acid (20:5n-3) were preferred substrates for hydrolysis using both fish oil and acyl-homogeneous TAGs with FFA as the main product of lipolysis. Hydrolysis of the natural fish oil TAG appeared slower yet produced proportionally more MAG and DAG after 5 min, and similar specificities, as for synthetic TAG substrates, were exhibited with 18:1n-9 and 20:5n-3 accumulating in the FFA fraction after 30 min. Notably, 16:0 was particularly conserved in MAG. As TAG resynthesis of absorbed lipid in salmon enterocytes proceeds preferably with 2-MAG as templates, the absorption of 2-MAG, produced during initial stages of TAG hydrolysis, would need to occur rapidly to be effectively utilised via the MAG pathway.
Authors:
A S Bogevik; A Oxley; R E Olsen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-21
Journal Detail:
Title:  Lipids     Volume:  43     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-09     Completed Date:  2008-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  655-62     Citation Subset:  IM    
Affiliation:
Institute of Marine Research, Matre Aquaculture Research Station, 5984, Matredal, Norway. andreb@imr.no
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MeSH Terms
Descriptor/Qualifier:
Animals
Fish Oils / chemistry*
Gastrointestinal Contents / chemistry*
Hydrolysis
Salmo salar*
Salts / chemistry
Tissue Extracts / chemistry*
Triglycerides / chemistry*
Chemical
Reg. No./Substance:
0/Fish Oils; 0/Salts; 0/Tissue Extracts; 0/Triglycerides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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