Document Detail

Hydroid TNF-receptor-associated factor (TRAF) and its splice variant: a role in development.
MedLine Citation:
PMID:  15163534     Owner:  NLM     Status:  MEDLINE    
TNF-receptor-associated factors (TRAFs) mediate signaling via tumor necrosis factor receptor (TNFR)/TLR molecules, playing a role in cellular processes such as growth, differentiation and apoptosis. They have been most studied in the immunological context. Within the animal kingdom, TRAFs have been characterized from vertebrates, flies and worms. We have cloned and characterized the first TRAF homologue from a member of the most basal eumetazoan phylum, the Cnidaria. The cnidarian TRAF, HyTRAF1, is a typical member of its family, containing one RING finger and five zinc finger domains in its N-terminal region, followed by a TRAF domain located at the C terminus. In addition to the full-length mRNA, the gene is alternatively spliced to create a shorter isoform, HyTRAF1a, with a deletion of 35 amino acids, resulting in a protein with only four zinc fingers. This is the first described TRAF alternative splicing in invertebrates. Whereas the full-length protein is expressed in most life stages of the animal, the short isoform is exclusively found at the larval and early metamorphic stages. This stage is characterized by extensive apoptosis, suggesting that HyTRAF1a mediates a proapoptotic c-jun N-terminal kinase (JNK) signaling, similar to the murine TRAF2A isoform. Based on neighbor joining analysis of TRAF molecules across the animal kingdom, we propose that the cnidarian TRAF interacts with TNFR, rather than with TLR. Our findings suggest that TNF signaling has evolved in the common ancestor to cnidarians and bilaterians and that it has been conserved in the entire animal kingdom.
Brahim Mali; Uri Frank
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular immunology     Volume:  41     ISSN:  0161-5890     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-27     Completed Date:  2004-08-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  377-84     Citation Subset:  IM    
Institute of Zoology, University of Heidelberg, INF 230, Heidelberg 69120, Germany.
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MeSH Terms
Alternative Splicing
Amino Acid Motifs
Amino Acid Sequence
Cnidaria / embryology,  genetics,  growth & development,  metabolism*
Evolution, Molecular
Invertebrates / metabolism
JNK Mitogen-Activated Protein Kinases
Metamorphosis, Biological
Mitogen-Activated Protein Kinases / physiology
Molecular Sequence Data
Protein Isoforms / physiology
Proteins / chemistry,  genetics,  isolation & purification,  physiology*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Homology, Amino Acid
Signal Transduction
Species Specificity
TNF Receptor-Associated Factor 1
Vertebrates / metabolism
Zinc Fingers / genetics
Reg. No./Substance:
0/Protein Isoforms; 0/Proteins; 0/TNF Receptor-Associated Factor 1; EC Mitogen-Activated Protein Kinases; EC Protein Kinases

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