| Hydrogen sulphide induced-dual vascular effect involves arachidonic acid cascade in rat mesenteric arterial bed. | |
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MedLine Citation:
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PMID: 21228064 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Hydrogen sulphide (H2S), a novel gaseous transmitter, is considered a physiological regulator of vascular homeostasis. Recent evidence suggests H2S as an endothelium hyperpolarizing factor (EDHF) candidate. To address this issue, we evaluated the vascular effect of sodium hydrogen sulphide (NaHS), an H2S donor on the rat mesenteric arterial bed. NaHS concentration-response curve was performed on pre-constricted mesenteric arterial bed. In order to asses the contribute of EDHF, we performed a pharmacological dissection using indomethacin, NG-nitro-L-arginine methyl ester (L-NAME) or apamin and charibdotoxin, as cyclooxygenase, nitric oxide-synthase and calcium-dependent potassium channel inhibitors, respectively. In another set of experiments we used 4-(4-Octadecylphenyl)-4-oxobutenoic acid, baicalein or proadifen as PLA2, lipoxygenase and P450 cytochrome inhibitors respectively. Finally, an immunofluorescence study was performed to support the involvement of PLA2 in mesenteric artery challenged by NaHS. NaHS promoted a dual vascular effect, i.e., vasoconstriction and vasodilation. L-NAME or baicalein administration affected neither NaHS-mediated vasodilation nor vasoconstriction, whereas apamin and charibdotoxin significantly inhibited NaHS-induced relaxation. Pre-treatment with PLA2 inhibitor abolished both the contracting and the relaxant effect, whereas P450 cytochrome blocker significantly reduced NaHS-mediated relaxation. The immunofluorescence study showed that NaHS caused a migration of cPLA2 close to the nucleus, which implies activation of this enzyme. Our data indicate that H2S could activate PLA2 that in turn releases arachidonic acid leading, initially, to vasoconstriction followed by vasodilation mediated by cytochrome P450-derived metabolites. Since EDHF has been presumed to be a cytochrome P450 derivative of the arachidonic acid, our results suggest that H2S acts through EHDF release. |
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Authors:
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Roberta d'Emmanuele di Villa Bianca; Rosalinda Sorrentino; Ciro Coletta; Emma Mitidieri; Antonietta Rossi; Valentina Vellecco; Aldo Pinto; Giuseppe Cirino; Raffaella Sorrentino |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-12 |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: - ISSN: 1521-0103 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 University of Naples; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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