Document Detail


Hydrogen sulfide regulates cAMP homeostasis and renin degranulation in As4.1 and rat renin-rich kidney cells.
MedLine Citation:
PMID:  21940660     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study aims to investigate the regulatory effect of hydrogen sulfide (H(2)S) on cAMP homeostasis and renin degranulation in As4.1 and rat renin-rich kidney cells. It was found in the present study that NaHS at 0.1-10 μM significantly decreased cAMP production in As4.1 cells treated with isoproterenol (a β-adrenoceptor agonist), forskolin (an adenylyl cyclase activator), or 3-isobutyl-1-methylxanthine (IBMX, a phosphodiesterase inhibitor). NaHS at 10 μM suppressed adenylate cyclase activity but stimulated phosphodiesterase activity. We continued to study whether H(2)S may mediate cAMP-dependent renin degranulaion in As4.1 cells. It was found that NaHS at 0.1-10 μM significantly increased intracellular renin protein level. Moreover, NaHS reversed the declined renin content within As4.1 cells and normalized the upregulated renin activity in the culture medium of As4.1 cells treated with the above three stimuli. RT-PCR showed that cystathionine-γ-lyase is the main enzyme to produce endogenous H(2)S in As4.1 cells. Overexpression of cystathionine-γ-lyase increased endogenous H(2)S production and suppressed isoproterenol-induced renin release, suggesting that endogenous H(2)S may also inhibit renin release from As4.1 cells. We also tested whether H(2)S has a similar effect in renin-rich kidney cells. It was found that isoproterenol elevated intracellular cAMP level and extracellular renin activity but decreased renin protein level in the renin-rich kidney cells. Pretreatment with NaHS abolished these effects. In conclusion, H(2)S regulates cAMP homeostasis via inhibition of adenylate cyclase and stimulation of phosphodiesterase. Our findings suggest that H(2)S plays a critical role in regulation of renin degranulation in As4.1 and rat renin-rich kidney cells.
Authors:
Ming Lu; Yi-Hong Liu; Chui Ying Ho; Chi Xin Tiong; Jin-Song Bian
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-09-21
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  302     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-19     Completed Date:  2012-02-06     Revised Date:  2012-10-26    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C59-66     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Degranulation / drug effects,  physiology*
Cyclic AMP / metabolism*,  physiology
Homeostasis / drug effects,  physiology*
Hydrogen Sulfide / pharmacology*
Kidney / cytology,  drug effects,  metabolism*
Male
Rats
Rats, Sprague-Dawley
Renin / metabolism*
Chemical
Reg. No./Substance:
60-92-4/Cyclic AMP; 7783-06-4/Hydrogen Sulfide; EC 3.4.23.15/Renin
Comments/Corrections
Comment In:
Am J Physiol Cell Physiol. 2012 Jan 1;302(1):C21-3   [PMID:  21998138 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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