Document Detail


Hydrogen sulfide protects H9c2 cells against doxorubicin-induced cardiotoxicity through inhibition of endoplasmic reticulum stress.
MedLine Citation:
PMID:  22203419     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The roles of hydrogen sulfide (H(2)S) and endoplasmic reticulum (ER) stress in doxorubicin (DOX)-induced cardiotoxicity are still unclear. This study aimed to dissect the hypothesis that H(2)S could protect H9c2 cells against DOX-induced cardiotoxicity by inhibiting ER stress. Our results showed that exposure of H9c2 cells to DOX significantly inhibited the expression and activity of cystathionine-γ-lyase (CSE), a synthetase of H(2)S, accompanied by the decreased cell viability and the increased reactive oxygen species (ROS) accumulation. In addition, exposure of cells to H(2)O(2) (an exogenous ROS) mimicked the inhibitory effect of DOX on the expression and activity of CSE. Pretreatment with N-acetyl-L: -cysteine (NAC) (a ROS scavenger) attenuated intracellular ROS accumulation, cytotoxicity, and the inhibition of expression and activity of CSE induced by DOX. Notably, the ER stress-related proteins, including glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) were obviously upregulated in DOX-treated H9c2 cells. Pretreatment with sodium hydrosulfide (NaHS, a H(2)S donor) before DOX exposure markedly suppressed DOX-induced overexpressions of GRP78 and CHOP, cytotoxicity and oxidative stress. In conclusion, we have demonstrated that ROS-mediated inhibition of CSE is involved in DOX-induced cytotoxicity in H9c2 cells, and that exogenous H(2)S can confer protection against DOX-induced cardiotoxicity partly through inhibition of ER stress.
Authors:
Xiu-Yu Wang; Chun-Tao Yang; Dong-Dan Zheng; Li-Qiu Mo; Ai-Ping Lan; Zhan-Li Yang; Fen Hu; Pei-Xi Chen; Xin-Xue Liao; Jian-Qiang Feng
Related Documents :
21766459 - Minimizing hydrodynamic stress in mammalian cell culture through the lobed taylor-couet...
1888139 - Intrinsic drug resistance in a human medullary thyroid carcinoma cell line: association...
2232889 - Concordant changes of pyrimidine metabolism in blasts of two cases of acute myeloid leu...
12062389 - Establishment and use of a cell line expressing hsv-1 thymidine kinase to characterize ...
8019989 - The development of drug resistance by tumor cells in vitro is accompanied by the develo...
7680349 - Decreased expression of the human folate receptor mediates transport-defective methotre...
22113439 - Protective effects of cleavage agents on ins-1 cells against h-iapp-induced apoptosis.
6368459 - Distribution of microtubules in cultured rpe cells from normal and dystrophic rcs rats.
20981869 - Caudatin-2,6-dideoxy-3-o-methy-β-d-cymaropyranoside 1 induced apoptosis through caspas...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-28
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  -     ISSN:  1573-4919     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adherence of stem cell transplant recipients receiving glucocorticoid therapy to an exercise-based r...
Next Document:  Function of the syndecan-4 cytoplasmic domain in oligomerization and association with ?-actinin in t...