Document Detail


Hydrogen sulfide decreases reactive oxygen in a model of lung transplantation.
MedLine Citation:
PMID:  22464394     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Ischemia-reperfusion injury is a common complication after lung transplantation. Ischemia-reperfusion injury is thought to be mediated by reactive oxygen species (ROS). Hydrogen sulfide (H(2)S) is a novel agent that has been previously shown to scavenge ROS and slow metabolism. We evaluated the effect of infused H(2)S on the presence of ROS after reperfusion in an ex vivo model of lung transplantation.
METHODS: Heart-Lung blocks were recovered from New Zealand white rabbits (n = 12) and cold stored in Perfadex solution for 18 h. After storage, the heart-lung blocks were reperfused ex vivo with donor rabbit blood. In the treatment group (n = 7), a bolus of sodium H(2)S was added at the beginning of reperfusion (100 μg/kg) and continuously infused throughout the 2-h experiment (1 mg/kg/h). The vehicle group (n = 5) received an equivalent volume of saline. Serial airway and pulmonary artery pressures and arterial and venous blood gases were measured.
RESULTS: Oxygenation and pulmonary artery pressures were similar between the 2 groups. However, treatment with H(2)S resulted in a dramatic reduction in the presence of ROS after 2 h of reperfusion (4,851 ± 2,139 versus 235 ± 462 related fluorescence units/mg protein; P = 0.003). A trend was seen toward increased levels of cyclic guanosine monophosphate in the H(2)S-treated group (3.08 ± 1.69 versus 1.73 ± 1.41 fmol/mg tissue; P = .23).
CONCLUSIONS: After prolonged ischemia, infusion of H(2)S during reperfusion was associated with a significant decrease in the presence of ROS, a suspected mediator of ischemia-reperfusion injury. To our knowledge, the present study represents the first reported therapeutic use of H(2)S in an experimental model of lung transplantation.
Authors:
Timothy J George; George J Arnaoutakis; Claude A Beaty; Simran K Jandu; Lakshmi Santhanam; Dan E Berkowitz; Ashish S Shah
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-18
Journal Detail:
Title:  The Journal of surgical research     Volume:  178     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-23     Completed Date:  2013-01-10     Revised Date:  2014-06-04    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  494-501     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure / physiology
Disease Models, Animal
Hydrogen Sulfide / pharmacology*
Lung Transplantation*
Perfusion
Pulmonary Artery / physiology
Pulmonary Wedge Pressure / physiology
Rabbits
Reactive Oxygen Species / metabolism*
Reperfusion Injury / drug therapy*,  metabolism*
Treatment Outcome
Grant Support
ID/Acronym/Agency:
R21 DK067210-02/DK/NIDDK NIH HHS; S10 RR027443-01/RR/NCRR NIH HHS; T32 2T32DK007713-12/DK/NIDDK NIH HHS; T32 CA126607/CA/NCI NIH HHS; T32 DK007713-12/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Reactive Oxygen Species; YY9FVM7NSN/Hydrogen Sulfide
Comments/Corrections

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