Document Detail


Hydrogen peroxide preconditioning protects PC12 cells against apoptosis induced by oxidative stress.
MedLine Citation:
PMID:  15830107     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Oxidative stress can induce significant cell death by apoptosis. We explore whether prior exposure to H2O2 (H2O2 preconditioning) protects PC12 cells against the apoptotic consequences of subsequent oxidative damages and what role the ATP-sensitive potassium (K(ATP)) channels play in the preconditioning protection. PC12 cells were preconditioned with 90 min exposure to H2O2 at 10 micromol/L, followed by 24-h recovery and subsequent exposures to different concentrations (20, 30, 50 and 100 micromol/L) of H2O2 for 24 h respectively. We used PI staining flow cytometry (FCM) to observe the apoptosis of PC12 cells. It was shown that 24-h exposures to H2O2 at 20, 30, 50 and 100 micromol/L respectively induced substantial cell apoptosis, which was greatly prevented in the preconditioning cells, indicating that H2O2 preconditioning protected PC12 cells against apoptosis induced by H2O2. Administration of pinacidil (10 micromol/L), an K(ATP) channel activator, significantly attenuated the apoptosis of PC12 cells induced by H2O2 at 30 and 50 micromol/L for 24 h respectively. Glybenclamide (10 micromol/L), a K(ATP) channel inhibitor, significantly suppressed or abolished the protective effects caused by the pinacidil but not by H2O2 preconditioning. However, when both H2O2 preconditioning and pinacidil were co-applied, their protection against the apoptosis of PC12 cells was much stronger than that of the individual one of them. These results suggest that H2O2 preconditioning protects PC12 cells against apoptosis and that the activation of K(ATP) channels is not involved in, but synergetically enhances adaptive protection of H2O2 preconditioning.
Authors:
Xiao-Qing Tang; Jing Chen; Er-Hu Tang; Jian-Qiang Feng; Pei-Xi Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Sheng li xue bao : [Acta physiologica Sinica]     Volume:  57     ISSN:  0371-0874     ISO Abbreviation:  Sheng Li Xue Bao     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-04-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730130R     Medline TA:  Sheng Li Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  211-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China.
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