Document Detail

Hydrogen peroxide functions as a secondary messenger for brassinosteroids-induced CO2 assimilation and carbohydrate metabolism in Cucumis sativus.
MedLine Citation:
PMID:  23024048     Owner:  NLM     Status:  MEDLINE    
Brassinosteroids (BRs) are potent regulators of photosynthesis and crop yield in agricultural crops; however, the mechanism by which BRs increase photosynthesis is not fully understood. Here, we show that foliar application of 24-epibrassinolide (EBR) resulted in increases in CO(2) assimilation, hydrogen peroxide (H(2)O(2)) accumulation, and leaf area in cucumber. H(2)O(2) treatment induced increases in CO(2) assimilation whilst inhibition of the H(2)O(2) accumulation by its generation inhibitor or scavenger completely abolished EBR-induced CO(2) assimilation. Increases of light harvesting due to larger leaf areas in EBR- and H(2)O(2)-treated plants were accompanied by increases in the photochemical efficiency of photosystem II (Φ(PSII)) and photochemical quenching coefficient (q(P)). EBR and H(2)O(2) both activated carboxylation efficiency of ribulose-1,5-bisphosphate oxygenase/carboxylase (Rubisco) from analysis of CO(2) response curve and in vitro measurement of Rubisco activities. Moreover, EBR and H(2)O(2) increased contents of total soluble sugar, sucrose, hexose, and starch, followed by enhanced activities of sugar metabolism such as sucrose phosphate synthase, sucrose synthase, and invertase. Interestingly, expression of transcripts of enzymes involved in starch and sugar utilization were inhibited by EBR and H(2)O(2). However, the effects of EBR on carbohydrate metabolisms were reversed by the H(2)O(2) generation inhibitor diphenyleneodonium (DPI) or scavenger dimethylthiourea (DMTU) pretreatment. All of these results indicate that H(2)O(2) functions as a secondary messenger for EBR-induced CO(2) assimilation and carbohydrate metabolism in cucumber plants. Our study confirms that H(2)O(2) mediates the regulation of photosynthesis by BRs and suggests that EBR and H(2)O(2) regulate Calvin cycle and sugar metabolism via redox signaling and thus increase the photosynthetic potential and yield of crops.
Yu-ping Jiang; Fei Cheng; Yan-hong Zhou; Xiao-jian Xia; Wei-hua Mao; Kai Shi; Zhi-xiang Chen; Jing-quan Yu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Zhejiang University. Science. B     Volume:  13     ISSN:  1862-1783     ISO Abbreviation:  J Zhejiang Univ Sci B     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-03-04     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101236535     Medline TA:  J Zhejiang Univ Sci B     Country:  China    
Other Details:
Languages:  eng     Pagination:  811-23     Citation Subset:  IM    
Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
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MeSH Terms
Brassinosteroids / pharmacology*
Carbohydrate Metabolism / drug effects,  genetics
Carbon Dioxide / metabolism
Chlorophyll / metabolism
Cucumis sativus / drug effects,  genetics,  growth & development,  metabolism*
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Hydrogen Peroxide / metabolism,  pharmacology
Photosynthesis / drug effects
Plant Growth Regulators / pharmacology
Reactive Oxygen Species / metabolism
Ribulose-Bisphosphate Carboxylase / metabolism
Second Messenger Systems
Seedling / drug effects,  growth & development,  metabolism
Steroids, Heterocyclic / pharmacology
Reg. No./Substance:
0/Brassinosteroids; 0/Plant Growth Regulators; 0/Reactive Oxygen Species; 0/Steroids, Heterocyclic; 124-38-9/Carbon Dioxide; 1406-65-1/Chlorophyll; 7722-84-1/Hydrogen Peroxide; EC Carboxylase; Y9IQ1L53OX/brassinolide

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