| Hydrogen peroxide functions as a secondary messenger for brassinosteroids-induced CO(2) assimilation and carbohydrate metabolism in Cucumis sativus. | |
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MedLine Citation:
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PMID: 23024048 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Brassinosteroids (BRs) are potent regulators of photosynthesis and crop yield in agricultural crops; however, the mechanism by which BRs increase photosynthesis is not fully understood. Here, we show that foliar application of 24-epibrassinolide (EBR) resulted in increases in CO(2) assimilation, hydrogen peroxide (H(2)O(2)) accumulation, and leaf area in cucumber. H(2)O(2) treatment induced increases in CO(2) assimilation whilst inhibition of the H(2)O(2) accumulation by its generation inhibitor or scavenger completely abolished EBR-induced CO(2) assimilation. Increases of light harvesting due to larger leaf areas in EBR- and H(2)O(2)-treated plants were accompanied by increases in the photochemical efficiency of photosystem II (Φ(PSII)) and photochemical quenching coefficient (q(P)). EBR and H(2)O(2) both activated carboxylation efficiency of ribulose-1,5-bisphosphate oxygenase/carboxylase (Rubisco) from analysis of CO(2) response curve and in vitro measurement of Rubisco activities. Moreover, EBR and H(2)O(2) increased contents of total soluble sugar, sucrose, hexose, and starch, followed by enhanced activities of sugar metabolism such as sucrose phosphate synthase, sucrose synthase, and invertase. Interestingly, expression of transcripts of enzymes involved in starch and sugar utilization were inhibited by EBR and H(2)O(2). However, the effects of EBR on carbohydrate metabolisms were reversed by the H(2)O(2) generation inhibitor diphenyleneodonium (DPI) or scavenger dimethylthiourea (DMTU) pretreatment. All of these results indicate that H(2)O(2) functions as a secondary messenger for EBR-induced CO(2) assimilation and carbohydrate metabolism in cucumber plants. Our study confirms that H(2)O(2) mediates the regulation of photosynthesis by BRs and suggests that EBR and H(2)O(2) regulate Calvin cycle and sugar metabolism via redox signaling and thus increase the photosynthetic potential and yield of crops. |
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Authors:
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Yu-Ping Jiang; Fei Cheng; Yan-Hong Zhou; Xiao-Jian Xia; Wei-Hua Mao; Kai Shi; Zhi-Xiang Chen; Jing-Quan Yu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of Zhejiang University. Science. B Volume: 13 ISSN: 1862-1783 ISO Abbreviation: J Zhejiang Univ Sci B Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101236535 Medline TA: J Zhejiang Univ Sci B Country: China |
Other Details:
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Languages: eng Pagination: 811-23 Citation Subset: IM |
Affiliation:
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Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Horticultural Plants Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China; Department of Botany and Plant Pathology, Purdue University, West Lafayette 47907-2054, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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