Document Detail


Hydrogen peroxide and corneal endothelium.
MedLine Citation:
PMID:  6798819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because of recent evidence of low levels of hydrogen peroxide in the aqueous humor, studies were performed to determine levels of corneal endothelial toxicity as well as factors modifying toxicity. Perfusion of cornea endothelial cells for 3 h with varying concentrations of hydrogen peroxide demonstrated a threshold of toxicity at a concentration between 0.3 and 0.5 mM H2O2. The toxic effect resulted in rapid corneal swelling as well as disruption of endothelial cell cytoplasm and organelles. Both the physiologic and anatomic toxic effects of 0.5 mM H2O2 could be blocked with 5400 U/ml catalase. Exposure of corneas to 20 mM H2O2 for 10 min in the presence of EDTA - Fe+3 resulted in an enhancement of corneal swelling rate more rapid than that which resulted from a 10 min exposure to 20 mM H2O2 alone. Neither the presence of ascorbic acid nor the absence of glutathione and adenosine had and effect on the cornea swelling rate which occurred during a 3 h perfusion of endothelium with 0.3 mM H2O2. Chelated iron had no effect on the corneal swelling induced by phototactivation of rose bengal presensitized cornea endothelial cells.
Authors:
D S Hull; S Csukas; K Green; V Livingston
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Acta ophthalmologica     Volume:  59     ISSN:  0001-639X     ISO Abbreviation:  Acta Ophthalmol (Copenh)     Publication Date:  1981 Jun 
Date Detail:
Created Date:  1982-03-22     Completed Date:  1982-03-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370347     Medline TA:  Acta Ophthalmol (Copenh)     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  409-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cornea / drug effects*
Edetic Acid / pharmacology
Endothelium / drug effects,  ultrastructure
Hydrogen Peroxide / toxicity*
Iron Chelating Agents / pharmacology
Rabbits
Grant Support
ID/Acronym/Agency:
EY01413U/EY/NEI NIH HHS; EY02386/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Iron Chelating Agents; 60-00-4/Edetic Acid; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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