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Hydrogen Sulfide Alleviates Myocardial Collagen Remodeling in Association with Inhibition of TGF-β/smad Signaling pathway in Spontaneously Hypertensive Rats.
MedLine Citation:
PMID:  25222913     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The study was designed to explore the role of hydrogen sulfide (H2S) in the regulation of myocardial collagen remodeling in spontaneously hypertensive rats (SHRs) and its possible mechanisms. We treated nine-week-old male SHRs and age- and sex-matched Wistar-Kyoto rats (WKYs) with NaHS (90 μmol·kg(-1)·day(-1)) for 9 weeks. At 18 weeks, plasma H2S, tail arterial pressure, morphology of the heart, myocardial ultrastructure and collagen volume fraction (CVF), myocardial expressions of collagen I and III protein and procollagen I and III mRNA, transforming growth factor-β1 (TGF-β1), TGF-β type I receptor (TβR-I), type II receptor (TβR-II), p-Smad2and 3, matrix metalloproteinase (MMP)-13 and tissue inhibitors of MMP (TIMP)-1 proteins were determined. TGF-β1-stimulated cultured cardiac fibroblasts (CFs) were used to further study the mechanisms. The results showed that compared with WKYs, SHRs showed a reduced plasma H2S, elevated tail artery pressure and increased myocardial collagen, TGF-β1, TβR-II, p-Smad2 and p-Smad3 expressions. However, NaHS markedly decreased tail artery pressure and inhibited myocardial collagen, TGF-β1, TβR-II, p-Smad2 and p-Smad3 protein expressions. But H2S had no effect on the expressions of MMP-13 and TIMP-1. Hydralazine reduced blood pressure, but had no effect on myocardial collagen, MMP-13 and TIMP-1 expressions and TGF-β1/Smad2/3 signaling pathway. H2S prevented activation of the TGF-β1/Smad2/3 signaling pathway and abnormal collagen synthesis in CFs. In conclusion, the results suggested that H2S could prevent myocardial collagen remodeling in SHR. The mechanism might be associated with inhibition of collagen synthesis via TGF-β1/Smad2/3 signaling pathway.
Authors:
Lili Sun; Hongfang Jin; Lujing Sun; Siyao Chen; Yaqian Huang; Jia Liu; Zhenzhen Li; Manman Zhao; Yan Sun; Chaoshu Tang; Bin Zhao; Junbao Du
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-10
Journal Detail:
Title:  Molecular medicine (Cambridge, Mass.)     Volume:  -     ISSN:  1528-3658     ISO Abbreviation:  Mol. Med.     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9501023     Medline TA:  Mol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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