Document Detail


Hydrogen peroxide-mediated growth of the root system occurs via auxin signaling modification and variations in the expression of cell-cycle genes in rice seedlings exposed to cadmium stress.
MedLine Citation:
PMID:  23013333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The link between root growth, H₂O₂, auxin signaling, and the cell cycle in cadmium (Cd)-stressed rice (Oryza sativa L. cv. Zhonghua No. 11) was analyzed in this study. Exposure to Cd induced a significant accumulation of Cd, but caused a decrease in zinc (Zn) content which resulted from the decreased expression of OsHMA9 and OsZIP. Analysis using a Cd-specific probe showed that Cd was mainly localized in the meristematic zone and vascular tissues. Formation and elongation of the root system were significantly promoted by 3-amino-1,2,4-triazole (AT), but were markedly inhibited by N,N'-dimethylthiourea (DMTU) under Cd stress. The effect of H₂O₂ on Cd-stressed root growth was further confirmed by examining a gain-of-function rice mutant (carrying catalase1 and glutathione-S-transferase) in the presence or absence of diphenylene iodonium. DR5-GUS staining revealed close associations between H₂O₂ and the concentration and distribution of auxin. H₂O₂ affected the expression of key genes, including OsYUCCA, OsPIN, OsARF, and OsIAA, in the auxin signaling pathway in Cd-treated plants. These results suggest that H₂O₂ functions upstream of the auxin signaling pathway. Furthermore, H₂O₂ modified the expression of cell-cycle genes in Cd-treated roots. The effects of H₂O₂ on root system growth are therefore linked to auxin signal modification and to variations in the expression of cell-cycle genes in Cd-stressed rice. A working model for the effects of H₂O₂ on Cd-stressed root system growth is thus proposed and discussed in this paper.
Authors:
Feng-Yun Zhao; Ming-Ming Han; Shi-Yong Zhang; Kai Wang; Cheng-Ren Zhang; Tao Liu; Wen Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-29
Journal Detail:
Title:  Journal of integrative plant biology     Volume:  54     ISSN:  1744-7909     ISO Abbreviation:  J Integr Plant Biol     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-12     Completed Date:  2013-05-17     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101250502     Medline TA:  J Integr Plant Biol     Country:  China (Republic : 1949- )    
Other Details:
Languages:  eng     Pagination:  991-1006     Citation Subset:  IM    
Copyright Information:
© 2012 Institute of Botany, Chinese Academy of Sciences.
Affiliation:
College of Life Sciences, Shandong University of Technology, Zibo 255049, China. zhaofengyun@sdut.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Cadmium / toxicity*
Genes, cdc*
Hydrogen Peroxide / pharmacology*
Indoleacetic Acids / metabolism*
Oryza sativa / cytology,  genetics*
Plant Roots / drug effects*,  growth & development,  metabolism
Signal Transduction / drug effects*
Chemical
Reg. No./Substance:
0/Indoleacetic Acids; 7440-43-9/Cadmium; 7722-84-1/Hydrogen Peroxide
Comments/Corrections
Erratum In:
J Integr Plant Biol. 2013 May;55(5):484

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