Document Detail


Hydrogels for lentiviral gene delivery.
MedLine Citation:
PMID:  23347508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Gene delivery from hydrogel biomaterials provides a fundamental tool for a variety of clinical applications including regenerative medicine, gene therapy for inherited disorders and drug delivery. The high water content and mild gelation conditions of hydrogels support their use for gene delivery by preserving activity of lentiviral vectors and acting to shield vectors from any host immune response.
AREAS COVERED: Strategies to control lentiviral entrapment within and retention/release from hydrogels are reviewed. The authors discuss the ability of hydrogel design parameters to control the transgene expression profile and the capacity of hydrogels to protect vectors from (and even modulate) the host immune response.
EXPERT OPINION: Delivery of genetic vectors from scaffolds provides a unique opportunity to capitalize on the potential synergy between the biomaterial design for cell processes and gene delivery. Hydrogel properties can be tuned to directly control the events that determine the tissue response to controlled gene delivery, which include the extent of cell infiltration, preservation of vector activity and vector retention. While some design parameters have been identified, numerous opportunities for investigation are available in order to develop a complete model relating the biomaterial properties and host response to gene delivery.
Authors:
Stephanie K Seidlits; Robert Michael Gower; Jaclyn A Shepard; Lonnie D Shea
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2013-01-25
Journal Detail:
Title:  Expert opinion on drug delivery     Volume:  10     ISSN:  1744-7593     ISO Abbreviation:  Expert Opin Drug Deliv     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-05-17     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  101228421     Medline TA:  Expert Opin Drug Deliv     Country:  England    
Other Details:
Languages:  eng     Pagination:  499-509     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Drug Delivery Systems
Gene Transfer Techniques*
Genetic Therapy
Genetic Vectors*
Humans
Hydrogels*
Lentivirus / genetics*
Transgenes
Grant Support
ID/Acronym/Agency:
F32 NS081961/NS/NINDS NIH HHS; PL1 EB008542/EB/NIBIB NIH HHS; PL1 EB008542/EB/NIBIB NIH HHS; R01 EB003806/EB/NIBIB NIH HHS; R01 EB003806/EB/NIBIB NIH HHS; R01 EB005678/EB/NIBIB NIH HHS; R01 EB005678/EB/NIBIB NIH HHS; R21 EB006520/EB/NIBIB NIH HHS; R21 EB006520/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Hydrogels
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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