Document Detail


Hydrodynamic shear stress to remove Listeria monocytogenes biofilms from stainless steel and polytetrafluoroethylene surfaces.
MedLine Citation:
PMID:  23043564     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIMS: To calculate the shear stress needed to remove sessile Listeria monocytogenes cells from stainless steel (SS) and polytetrafluoroethylene (PTFE) surfaces. METHODS AND RESULTS: L. monocytogenes biofilms were formed on SS and PTFE surfaces. Shear stress was calculated using a radial flow chamber device and cells quantified by staining with DAPI. Results showed that shear stress between 24 and 144 N m(-2) removed up to 98% of cells from SS surfaces. PTFE presents a very hydrophobic surface and a significant lower removal (P < 0.05) of only 63% removal was achieved; moreover, on PTFE discs detachment of L. monocytogenes biofilms was more efficient at a lower shear stress (between 8.6 and 34 N m(-2) ). CONCLUSIONS: Water flow is more effective in removing L. monocytogenes biofilms from SS surfaces than from PTFE materials. SIGNIFICANCE AND IMPACT OF THE STUDY: This work clearly demonstrates that water flow does not have the same efficiency in removing cells from different material surfaces and shows the need to optimize cleaning and sampling procedures by considering the conditions in which cells attach to surfaces and the physico-chemistry of the surfaces. © 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Maria S Gião; Charles W Keevil
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Centre for Biological Sciences, Highfield Campus, Life Sciences Building, University of Southampton, Southampton, SO17 1BJ, U.K.
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