Document Detail


Hydrocortisone reinforces the blood-brain properties in a serum free cell culture system.
MedLine Citation:
PMID:  9679029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The increasing number of newly developed drugs demands for functional in vitro models of the blood-brain barrier to determine their brain uptake. Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to the in vivo situation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 Omega x cm2. We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 Omega x cm2 were measured in the presence of physiological relevant hydrocortisone concentrations. In correspondence to the TER increase hydrocortisone decreased cell monolayer permeability for sucrose down to 5x10(-7) cm/s, which is close to the in vivo value of 1.2x10(-7) cm/s and by a factor of five lower compared to cultures without hydrocortisone and in presence of serum.
Authors:
D Hoheisel; T Nitz; H Franke; J Wegener; A Hakvoort; T Tilling; H J Galla
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Publication Detail:
Type:  Corrected and Republished Article; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  247     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-20     Completed Date:  1998-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  312-5     Citation Subset:  IM    
Affiliation:
Institut für Biochemie, Westfälische Wilhelms-Universität, Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Brain Barrier / drug effects*,  physiology
Capillaries / cytology,  drug effects,  physiology
Capillary Permeability / drug effects
Capillary Resistance / drug effects
Cell Culture Techniques / methods*
Cerebral Cortex / blood supply,  cytology
Culture Media, Serum-Free
Drug Evaluation, Preclinical
Endothelium, Vascular / cytology,  drug effects,  physiology
Epidermal Growth Factor / pharmacology
Hydrocortisone / pharmacology*
Insulin / pharmacology
Models, Biological
Sucrose / pharmacokinetics
Swine
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 11061-68-0/Insulin; 50-23-7/Hydrocortisone; 57-50-1/Sucrose; 62229-50-9/Epidermal Growth Factor
Comments/Corrections
Corrected and Republished From:
Biochem Biophys Res Commun. 1998 Mar 6;244(1):312-6   [PMID:  9514852 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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