Document Detail

Hydrocortisone reinforces the blood-brain barrier properties in a serum free cell culture system.
MedLine Citation:
PMID:  9514852     Owner:  NLM     Status:  MEDLINE    
The increasing number of newly developed drugs demands for functional in vitro models of the blood-brain barrier to determine their brain uptake. Cultured cerebral capillary endothelial cells are considered to be such a model, however in serum containing media they exhibit low electrical resistances and high permeabilities compared to the in vivo situation. Here we report the establishment of a serum-free cell culture model. Withdrawal of serum already caused a twofold increase of transendothelial resistance (TER), which in presence of serum is about 100-150 omega.cm2. We tested several supplements and found that hydrocortisone is a potent stimulator for the formation of barrier properties. TERs up to 1000 omega.cm2 were measured in the presence of physiological relevant hydrocortisone concentrations. In correspondence to the TER increase hydrocortisone decreased cell monolayer permeability for sucrose down to 5 x 10(-7) cm/s, which is close to the in vivo value of 1.2 x 10(-7) cm/s and by a factor of five lower compared to cultures without hydrocortisone and in presence of serum.
D Hoheisel; T Nitz; H Franke; J Wegener; A Hakvoort; T Tilling; H J Galla
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  244     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-09     Completed Date:  1998-04-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  312-6     Citation Subset:  IM    
Institut für Biochemie, Westfälische Wilhelms-Universität, Münster, Germany.
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MeSH Terms
Blood-Brain Barrier / drug effects*
Capillary Permeability / drug effects
Capillary Resistance / drug effects
Cell Culture Techniques / methods*
Cerebral Cortex / blood supply,  cytology*
Culture Media, Serum-Free
Endothelium, Vascular / drug effects
Epidermal Growth Factor / pharmacology
Hydrocortisone / pharmacology*
Insulin / pharmacology
Models, Biological
Sucrose / metabolism
Reg. No./Substance:
0/Culture Media, Serum-Free; 11061-68-0/Insulin; 50-23-7/Hydrocortisone; 57-50-1/Sucrose; 62229-50-9/Epidermal Growth Factor
Corrected and Republished In:
Biochem Biophys Res Commun. 1998 Jun 18;247(2):312-5   [PMID:  9679029 ]

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