Document Detail


Hydrocortisone is associated with cell surface blebbing in cultures of human embryonic lung fibroblasts.
MedLine Citation:
PMID:  3796057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Treatment with hydrocortisone (HC) is associated with increased cell surface blebbing of proliferatively active WI-38 and IMR-90 fibroblasts at low and high population doubling levels within the first 24 h after seeding. Time course studies show a marked decrease in blebbing after 2 days. In contrast, very old cells near the end of the population life span (greater than 96% life span completed) show blebbing which is independent of HC treatment. Since the time of increased HC-associated blebbing in cycling cell populations coincides with the time during which HC-treated cells condition the medium with a growth-stimulating (autocrine) factor, it is possible that blebbing may represent extrusion of this factor from the cells at these times.
Authors:
B M Stanulis-Praeger; M A Shatos; V J Cristofalo
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  36     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-02-17     Completed Date:  1987-02-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  243-58     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging / physiology
Cell Line
Cell Membrane / drug effects*
Embryo, Mammalian
Fibroblasts / drug effects*,  ultrastructure
Humans
Hydrocortisone / pharmacology*
Microscopy, Electron, Scanning
Mitosis
Organoids / ultrastructure
Grant Support
ID/Acronym/Agency:
AG00378/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
50-23-7/Hydrocortisone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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