| Hydrochloric vs. sulphuric acid in water for Ziehl-Neelsen staining of acid-fast bacilli. | |
| | |
MedLine Citation:
|
PMID: 21682971 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
SETTING: Damien Foundation Bangladesh tuberculosis (TB) control projects. OBJECTIVES: To compare 25% sulphuric acid in water (H(2)SO(4)) with hydrochloric acid in water (HCl) to differentiate acid-fast bacilli in sputum smears stained with 1% carbolfuchsin. DESIGN: For 1 year, all 158 microscopy laboratories used either H(2)SO(4) or 3%/6%/10% HCl for their routine work, alternating monthly between H(2)SO(4) and HCl. Each month a sample of five smears per laboratory was rechecked blind. After recording qualitative staining aspects, all sample smears were restained before rechecking, using H(2)SO(4) for destaining. RESULTS: A total of 368 059 H(2)SO(4) and 335 436 HCl smears were routinely read, yielding 7.2% positive or scanty results in both groups. Of these, 9492 were rechecked. There was no difference in false-negatives detected (0.66%, 95%CI 0.44-0.95 for H(2)SO(4) vs. 0.68%, 95%CI 0.46-0.98 for HCl), but apparently there were more false-positives with H(2)SO(4) (2.12%, 95%CI 0.92-4.14 vs. 0.28%, 95%CI 0.00-1.54, P = 0.05). Qualitatively, only 3% HCl yielded significantly inferior differentiation results. CONCLUSIONS: HCl 6-10% in water can be recommended for Ziehl-Neelsen destaining above H(2)SO(4). Diluting is easier and safer, and it may cause less confusion with false-positives during rechecking, including a restaining step. |
| | |
Authors:
|
K J M Aung; P Nandi; A Hamid Salim; A Hossain; A Van Deun |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Volume: 15 ISSN: 1815-7920 ISO Abbreviation: Int. J. Tuberc. Lung Dis. Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-06-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9706389 Medline TA: Int J Tuberc Lung Dis Country: France |
Other Details:
|
Languages: eng Pagination: 955-8 Citation Subset: IM |
Affiliation:
|
Damien Foundation Bangladesh, Dhaka, Bangladesh. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Early bactericidal activity of delamanid (OPC-67683) in smear-positive pulmonary tuberculosis patien...
Next Document: Evaluation of the Genotype® MTBDRplus assay as a tool for drug resistance surveys.