Document Detail


Hydrocarbon synthesis by enzymatically dissociated oenocytes of the abdominal integument of the German Cockroach, Blattella germanica.
MedLine Citation:
PMID:  12649753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In insects, hydrocarbons waterproof the cuticle, protect the insect from the external environment, and serve as semiochemicals or their metabolic precursors. In the German cockroach, Blattella germanica, hydrocarbons are synthesized by the abdominal integument, but the precise site of biosynthesis is not known. We developed a method for separation of oenocytes from other cells in the abdominal integument using enzymatic dissociation followed by Percoll gradient centrifugation. Radiolabeled propionate was then used to monitor de novo synthesis of hydrocarbons by dissociated cells. Oenocyte-enriched cell suspensions of abdominal sternites synthesized hydrocarbons, whereas suspensions enriched with epidermal cells did not. Our results show conclusively that hydrocarbons are produced by oenocytes not only in insects whose oenocytes are localized within the hemocoel, but also in those insects whose oenocytes are within the abdominal integument. Furthermore, these data support a hemolymph pathway for transport and delivery of hydrocarbons to both external and internal tissues, including the epicuticle, fat body, and ovaries.
Authors:
Yongliang Fan; Ludek Zurek; Michael J Dykstra; Coby Schal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2003-02-13
Journal Detail:
Title:  Die Naturwissenschaften     Volume:  90     ISSN:  0028-1042     ISO Abbreviation:  Naturwissenschaften     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-21     Completed Date:  2003-06-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400767     Medline TA:  Naturwissenschaften     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  121-6     Citation Subset:  IM    
Affiliation:
Department of Entomology and WM Keck Center for Behavioral Biology, North Carolina State University, Box 7613, Raleigh, NC 27695-7613, USA.
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MeSH Terms
Descriptor/Qualifier:
Abdomen
Animals
Blattellidae / enzymology*,  ultrastructure
Hydrocarbons / metabolism*
Microscopy, Electron
Chemical
Reg. No./Substance:
0/Hydrocarbons

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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